ArticleInternational endodontic journal2022
Multispecies biofilm removal by a multisonic irrigation system in mandibular molars.
Article in International endodontic journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT06959602 (Antibacterial Efficacy and Outcomes of Root Canal Irrigation Methods), which is not on this map. Cited by 14 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Antibacterial Efficacy and Outcomes of Root Canal Irrigation Methods
Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it, 25 citations in OpenAlex.
- Effectiveness of the GentleWave system in root canal disinfection: a systematic review.BMC oral health · 2026Pooled it
- A retrospective pragmatic clinical study of healing and predictors of success following gentlewave and conventional endodontic irrigation.Clinical oral investigations · 2026Article
- An Assessment of the Antibacterial Efficacy of the GentleWave System in Comparison to Other Endodontic Irrigation Systems: A Systematic Review.Clinical and experimental dental research · 2026Article
- Multispecies Biofilms Treated With Endodontic Sealers or Calcium Hydroxide: Antimicrobial Activity and Changes in Community Composition.International endodontic journal · 2025Article
- Bibliometric analysis of the GentleWave system: trends, collaborations, and research gaps.Restorative dentistry & endodontics · 2025Article
- Review
- Dissolution of Bovine Palatal Tissue with Degassed Sodium Hypochlorite.Dentistry journal · 2025Article
- Endodontic bioceramics: current and futurity aspects.Frontiers in oral health · 2025Review
- An In Vitro Evaluation of the Antimicrobial Efficacy of a Novel Irrigant Using Next-Generation Sequencing.Journal of endodontics · 2024Article
- Article
- Resistance, Tolerance, Virulence and Bacterial Pathogen Fitness-Current State and Envisioned Solutions for the Near Future.Pathogens (Basel, Switzerland) · 2023Review
- Bioceramics in Endodontics: Updates and Future Perspectives.Bioengineering (Basel, Switzerland) · 2023Review
- Multispecies biofilm removal by a multisonic irrigation system in mandibular molars.International endodontic journal · 2022Article
- Effect of different disinfection protocols in bacterial viability of an intraradicular biofilm formed in situ.Brazilian dental journalArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
aimThe aim of the study was to assess biofilm removal efficacy of GentleWave System and passive ultrasonic irrigation (PUI). METHODOLOGY: Twenty-two human mandibular molars with Vertucci's type II configuration in the mesial root were selected. Teeth were autoclaved, inoculated with dental plaque and incubated in a CDC biofilm reactor for two weeks. The mesial roots were instrumented up to 20.06 file (V-Taper) for the GentleWave group and up to 35.04 file (Vortex Blue) for PUI group. Irrigation was performed using GentleWave and PUI irrigation protocols (n = 11). Dentine debris on paper points samples were obtained for quantitative real-time polymerase chain reaction (qPCR) and 16S ribosomal RNA gene sequencing (next-generation aequencing-NGS). For qPCR, a non-parametric test (α = 0.05) was used. Next-generation sequencing data were analysed using mothur, with alpha diversity calculated as the Shannon and Chao1 indices and Bray-Curtis dissimilarities were used for beta diversity. Differences in alpha diversity and abundances of genera were evaluated using Kruskal-Wallis test. Differences in community composition were evaluated using analysis of similarity with Bonferroni correction for multiple comparisons.
resultsQuantitative real-time polymerase chain reaction results showed that the reduction estimated in percentages for both groups was equivalent (p > .05). NGS analysis showed that both techniques promoted a significant reduction in reads and OTUs number (p < .05). Shannon alpha diversity and Chao1 index showed no differences between pre- or post-treatment samples for both groups (p > .05). Additionally, pre-treatment communities differed from post-treatment samples in both groups regarding bacterial taxa reduction (ANOSIM R = 0.50 and 0.55, p < .001).
conclusionsBacterial reduction in mesial roots of mandibular molars prepared to 35.04 with PUI was similar to those prepared to 20.06 with a multisonic irrigant activation system.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.