Evidence map›Paper›PMID 35975649›Full record

ArticleOpen biology2022

Optogenetic manipulation of cyclic guanosine monophosphate to probe phosphodiesterase activities in megakaryocytes.

Yujing Zhang, Pascal Benz, Daniel Stehle, Shang Yang, Hendrikje Kurz, Susanne Feil, Georg Nagel, Robert Feil, Shiqiang Gao, Markus Bender

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Open biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Yujing ZhangInstitute of Experimental Biomedicine-Chair I, University Hospital and Rudolf Virchow Center, 97080 Würzburg, Germany.
Pascal BenzInstitute of Experimental Biomedicine-Chair I, University Hospital and Rudolf Virchow Center, 97080 Würzburg, Germany.
Daniel StehleInterfakultäres Institut für Biochemie, University of Tübingen, Tübingen, Baden-Württemberg, Germany.
Shang YangDepartment of Neurophysiology, Institute of Physiology, Biocenter, University of Würzburg, 97070 Würzburg, Germany.
Hendrikje KurzInstitute of Experimental Biomedicine-Chair I, University Hospital and Rudolf Virchow Center, 97080 Würzburg, Germany.
Susanne FeilInterfakultäres Institut für Biochemie, University of Tübingen, Tübingen, Baden-Württemberg, Germany.
Georg NagelDepartment of Neurophysiology, Institute of Physiology, Biocenter, University of Würzburg, 97070 Würzburg, Germany.
Robert FeilInterfakultäres Institut für Biochemie, University of Tübingen, Tübingen, Baden-Württemberg, Germany.ORCID 0000-0002-7335-4841
Shiqiang GaoDepartment of Neurophysiology, Institute of Physiology, Biocenter, University of Würzburg, 97070 Würzburg, Germany.ORCID 0000-0001-6190-9443
Markus BenderInstitute of Experimental Biomedicine-Chair I, University Hospital and Rudolf Virchow Center, 97080 Würzburg, Germany.ORCID 0000-0002-2381-116X
University of Tübingen · DEUniversity of Würzburg · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cyclic guanosine monophosphate (cGMP) signalling plays a fundamental role in many cell types, including platelets. cGMP has been implicated in platelet formation, but mechanistic detail about its spatio-temporal regulation in megakaryocytes (MKs) is lacking. Optogenetics is a technique which allows spatio-temporal manipulation of molecular events in living cells or organisms. We took advantage of this method and expressed a photo-activated guanylyl cyclase,

Indexed as

Guanosine MonophosphateMegakaryocytesBlastocladiellaCyclic GMPNitric OxideOptogeneticsPhosphoric Diester HydrolasesCyclic GMPGuanosine MonophosphateNitric OxidePhosphoric Diester HydrolasescGMPmegakaryocytesoptogeneticsphosphodiesterase

Identifiers

PMID35975649
PMCPMC9382455
OpenAlexW4292266650

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.