ArticleScientific reports2022
An automated 3D-printed perfusion bioreactor combinable with pulsed electromagnetic field stimulators for bone tissue investigations.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 34 citations in OpenAlex.
- From microtissues to macro solutions - The future of scalable and automated cartilage tissue engineering.Journal of orthopaedic translation · 2026Review
- Design and validation of a perfusion bioreactor for long-term human bone explant culture.JBMR plus · 2026Article
- Advanced Biomaterial-Based In Vitro Osteoarthritis Models: Integrating Sex as a Biological Variable in Hormonal, Subchondral Bone, and Mechanobiological Pathways.Journal of functional biomaterials · 2026Review
- Modeling and targeting the hostile physicochemical niche in bone metastasis: from experimental platforms to niche-directed therapy.Frontiers in cell and developmental biology · 2026Review
- A Modular Perfusion Bioreactor Platform for Simulating Bone Regeneration and Fracture Healing: Integrating Mechanical Loading and Dual Perfusion for Advanced In Vitro Models.Advanced healthcare materials · 2025Article
- Novel Nanomaterials for Developing Bone Scaffolds and Tissue Regeneration.Nanomaterials (Basel, Switzerland) · 2025Review
- Development and deployment of a functional 3D-bioprinted blood vessel.Scientific reports · 2025Article
- Fabrication of a novel 3D-printed perfusion bioreactor for complex cell culture models.Scientific reports · 2025Article
- Insights into bone and cartilage responses to pulsed electromagnetic field stimulation: a review with quantitative comparisons.Frontiers in bioengineering and biotechnology · 2025Review
- A bioreactor-based platform for investigating the early response of human periodontal ligament stem cells to intermittent mechanical stretching.Frontiers in bioengineering and biotechnology · 2025Article
- 3D-printed constructs deliver bioactive cargos to expedite cartilage regeneration.Journal of pharmaceutical analysis · 2024Review
- Article
- Effect ofInternational journal of molecular sciences · 2024Article
- Adaptable test bench for ASTM-compliant permeability measurement of porous scaffolds for tissue engineering.Scientific reports · 2024Article
- Bone Regeneration Revolution: Pulsed Electromagnetic Field Modulates Macrophage-Derived Exosomes to Attenuate Osteoclastogenesis.International journal of nanomedicine · 2024Article
- A novel perfusion bioreactor promotes the expansion of pluripotent stem cells in a 3D-bioprinted tissue chamber.Biofabrication · 2023Article
- Computer-aided engineering and additive manufacturing for bioreactors in tissue engineering: State of the art and perspectives.Biophysics reviews · 2023Review
- Review
- Article
- Challenges of Periodontal Tissue Engineering: Increasing Biomimicry through 3D Printing and Controlled Dynamic Environment.Nanomaterials (Basel, Switzerland) · 2022Review
Corrections and comments
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Authors and funding
15 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In bone tissue engineering research, bioreactors designed for replicating the main features of the complex native environment represent powerful investigation tools. Moreover, when equipped with automation, their use allows reducing user intervention and dependence, increasing reproducibility and the overall quality of the culture process. In this study, an automated uni-/bi-directional perfusion bioreactor combinable with pulsed electromagnetic field (PEMF) stimulation for culturing 3D bone tissue models is proposed. A user-friendly control unit automates the perfusion, minimizing the user dependency. Computational fluid dynamics simulations supported the culture chamber design and allowed the estimation of the shear stress values within the construct. Electromagnetic field simulations demonstrated that, in case of combination with a PEMF stimulator, the construct can be exposed to uniform magnetic fields. Preliminary biological tests on 3D bone tissue models showed that perfusion promotes the release of the early differentiation marker alkaline phosphatase. The histological analysis confirmed that perfusion favors cells to deposit more extracellular matrix (ECM) with respect to the static culture and revealed that bi-directional perfusion better promotes ECM deposition across the construct with respect to uni-directional perfusion. Lastly, the Real-time PCR results of 3D bone tissue models cultured under bi-directional perfusion without and with PEMF stimulation revealed that the only perfusion induced a ~ 40-fold up-regulation of the expression of the osteogenic gene collagen type I with respect to the static control, while a ~ 80-fold up-regulation was measured when perfusion was combined with PEMF stimulation, indicating a positive synergic pro-osteogenic effect of combined physical stimulations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.