ArticleScientific reports2022
High-throughput barcoding method for the genetic surveillance of insecticide resistance and species identification in Anopheles gambiae complex malaria vectors.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 32 citations in OpenAlex.
- Trial
- Operationalising Environmental DNA (eDNA) Detection of Major Malaria Vector Species in Ghana.Molecular ecology resources · 2026Article
- A targeted amplicon sequencing panel for cost-effective high-throughput genotyping of Aedes aegypti.Parasites & vectors · 2026Article
- Population genomics ofScience (New York, N.Y.) · 2026Article
- Enhancing whole genome DNA amplification of Plasmodium falciparum for advanced molecular surveillance in malaria control.Malaria journal · 2025Article
- Strengthening research and training on insecticide resistance in arthropod vectors in South America: The WINSA network.PLoS neglected tropical diseases · 2025Article
- Bulk identification of Anophelinae larvae from fishponds using DNA metabarcoding.Acta tropica · 2025Article
- A targeted amplicon sequencing panel for cost-effective high-throughput genotyping ofbioRxiv : the preprint server for biology · 2025Article
- A Short-Read Amplicon Sequencing Protocol and Bioinformatic Pipeline for Ecological Surveillance of Dipteran Disease Vectors.Molecular ecology resources · 2025Article
- Novel insecticide resistance mutations associated with variable PBO synergy in Anopheles gambiae s.l. from the Democratic Republic of Congo.Scientific reports · 2025Article
- Profiling insecticide resistance phenotypes and genotypes in Aedes aegypti populations across four regions in Puerto Rico.Scientific reports · 2025Article
- Preferential transmission of minority and drug-resistant clones in polyclonal infections in Mali.Malaria journal · 2025Article
- Omics Approaches in Understanding Insecticide Resistance in Mosquito Vectors.International journal of molecular sciences · 2025Review
- Application of a targeted amplicon sequencing panel to screen for insecticide resistance mutations in Anopheles darlingi populations from Brazil.Scientific reports · 2025Article
- The application of DNA markers in population genetics of mosquitoes: a comprehensive review.Frontiers in insect science · 2025Review
- Phenotypic evidence of deltamethrin resistance and identification of selective sweeps in Anopheles mosquitoes on the Bijagós Archipelago, Guinea-Bissau.Scientific reports · 2024Article
- Whole genome sequence analysis of population structure and insecticide resistance markers in Anopheles melas from the Bijagós Archipelago, Guinea-Bissau.Parasites & vectors · 2024Article
- Considerations for first field trials of low-threshold gene drive for malaria vector control.Malaria journal · 2024Review
- Pathogen prospecting of museums: Reconstructing malaria epidemiology.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Genomic surveillance of Anopheles mosquitoes on the Bijagós Archipelago using custom targeted amplicon sequencing identifies mutations associated with insecticide resistance.Parasites & vectors · 2024Article
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Authors and funding
22 authors at 6 institutions in 6 countries.
Funding
Abstract
Surveillance of malaria vector species and the monitoring of insecticide resistance are essential to inform malaria control strategies and support the reduction of infections and disease. Genetic barcoding of mosquitoes is a useful tool to assist the high-throughput surveillance of insecticide resistance, discriminate between sibling species and to detect the presence of Plasmodium infections. In this study, we combined multiplex PCR, custom designed dual indexing, and Illumina next generation sequencing for high throughput single nucleotide polymorphism (SNP)-profiling of four species from the Anopheles (An.) gambiae complex (An. gambiae sensu stricto, An. coluzzii, An. arabiensis and An. melas). By amplifying and sequencing only 14 genetic fragments (500 bp each), we were able to simultaneously detect Plasmodium infection; insecticide resistance-conferring SNPs in ace1, gste2, vgsc and rdl genes; the partial sequences of nuclear ribosomal internal transcribed spacers (ITS1 and ITS2) and intergenic spacers (IGS), Short INterspersed Elements (SINE), as well as mitochondrial genes (cox1 and nd4) for species identification and genetic diversity. Using this amplicon sequencing approach with the four selected An. gambiae complex species, we identified a total of 15 non-synonymous mutations in the insecticide target genes, including previously described mutations associated with resistance and two new mutations (F1525L in vgsc and D148E in gste2). Overall, we present a reliable and cost-effective high-throughput panel for surveillance of An. gambiae complex mosquitoes in malaria endemic regions.
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