ArticleElectrophoresis2023
Rapid isolation and quantification of extracellular vesicles from suspension-adapted human embryonic kidney cells using capillary-channeled polymer fiber spin-down tips.
Article in Electrophoresis, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 15 citations in OpenAlex.
- Comparison of polyester (PET) capillary-channeled polymer (C-CP) fiber spin-down tips and size exclusion chromatography (SEC) for the isolation of HEK 293-derived extracellular vesicles (EVs).Analytical and bioanalytical chemistry · 2026Article
- Comparison of light scattering-based detection methods for the sizing and number density characterization of extracellular vesicles (EV) isolated from human embryonic kidney (HEK) cell cultures.Analytical and bioanalytical chemistry · 2026Article
- Hydrophobic interaction chromatography resolves extracellular vesicle fractions with distinct lipidomic signatures.Scientific reports · 2026Article
- Cholesteryl esters and high protein-to-lipid ratios distinguish non-vesicular extracellular particles from extracellular vesicles.Cell communication and signaling : CCS · 2026Article
- Rapid method for the high purity isolation of bovine milk-derived extracellular vesicles via polyester (PET) capillary-channeled polymer (C-CP) fiber columns.Nanotheranostics · 2026Article
- A Rapid, High-Resolution Chromatographic Method for Isolating Subpopulations of HEK293-derived Extracellular Vesicles.Nanotheranostics · 2026Article
- Hyaluronic acid-ornithine crosslinked hydrogel as a superior 3D culture platform for high-quality exosome production in advanced wound healing.Stem cell research & therapy · 2025Article
- Isolation of Urinary Extracellular Vesicles (EVs) via Hydrophobic Interaction Chromatography Using a Nylon-6 Capillary-Channeled Polymer (C-CP) Fiber Column.Journal of separation science · 2025Article
- Isolation and quantification of human urinary exosomes using a Tween-20 elution solvent from polyester, capillary-channeled polymer fiber columns.Analytica chimica acta · 2024Article
- In-line coupling of capillary-channeled polymer fiber columns with optical absorbance and multi-angle light scattering detection for the isolation and characterization of exosomes.Analytical and bioanalytical chemistry · 2024Article
- Exploring capabilities of elemental mass spectrometry for determination of metal and biomolecules in extracellular vesicles.Analytical and bioanalytical chemistry · 2024Article
- Quantitative Recoveries of Exosomes and Monoclonal Antibodies from Chinese Hamster Ovary Cell Cultures by Use of a Single, Integrated Two-Dimensional Liquid Chromatography Method.Analytical chemistry · 2023Article
- Rapid isolation and quantification of extracellular vesicles from suspension-adapted human embryonic kidney cells using capillary-channeled polymer fiber spin-down tips.Electrophoresis · 2023Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Exosomes, a subset of extracellular vesicles (EVs, 30-200-nm diameter), serve as biomolecular snapshots of their cell of origin and vehicles for intercellular communication, playing roles in biological processes, including homeostasis maintenance and immune modulation. The large-scale processing of exosomes for use as therapeutic vectors has been proposed, but these applications are limited by impure, low-yield recoveries from cell culture milieu (CCM). Current isolation methods are also limited by tedious and laborious workflows, especially toward an isolation of EVs from CCM for therapeutic applications. Employed is a rapid (<10 min) EV isolation method on a capillary-channeled polymer fiber spin-down tip format. EVs are isolated from the CCM of suspension-adapted human embryonic kidney cells (HEK293), one of the candidate cell lines for commercial EV production. This batch solid-phase extraction technique allows 10
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.