Evidence map›Paper›PMID 35972968›Full record

ArticlePloS one2022

The potential role of serum expression profile of long non coding RNAs, Cox2 and HOTAIR as novel diagnostic biomarkers in systemic lupus erythematosus.

Rania H Mahmoud, Nermeen A Fouad, Enas M Hefzy, Olfat G Shaker, Tarek I Ahmed, Hoda A Hussein, Maha H Nasr, Othman M Zaki, Noha K Abdelghaffar, Omayma O Abdelaleem

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.9field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 11 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Rania H MahmoudDepartment of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Fayoum University, Fayoum, Egypt.ORCID 0000-0002-6876-992X
Nermeen A FouadDepartment of Rheumatology and Rehabilitation, Faculty of Medicine, Fayoum University, Fayoum, Egypt.
Enas M HefzyDepartment of Medical Microbiology and Immunology, Faculty of Medicine, Fayoum University, Cairo, Egypt.ORCID 0000-0001-9363-3998
Olfat G ShakerDepartment of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt.
Tarek I AhmedDepartment of Internal Medicine, Faculty of Medicine, Fayoum University, Fayoum, Egypt.
Hoda A HusseinDepartment of Internal Medicine, Faculty of Medicine, Fayoum University, Fayoum, Egypt.
Maha H NasrDepartment of Rheumatology and Rehabilitation, Faculty of Medicine, Fayoum University, Fayoum, Egypt.
Othman M ZakiDepartment of Clinical Pathology, Faculty of Medicine, Damietta University, Damietta, Egypt.
Noha K AbdelghaffarDepartment of Clinical Pathology, Faculty of Medicine, Fayoum University, Fayoum, Egypt.
Omayma O AbdelaleemDepartment of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Fayoum University, Fayoum, Egypt.
Fayoum University · EGCairo University · EGDamietta University · EG

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe role of the long non-coding RNAs (lncRNAs) in the pathogenesis of systemic lupus erythematosus (SLE) is mostly unknown, despite increasing evidence that lncRNAs extensively participate in physiological and pathological conditions.

aimTo detect the level of lncRNA-Cox2, HOTAIR, IL-6, and MMP-9 in the serum of SLE patients and to correlate these levels with disease activity and patients' clinical and laboratory data to evaluate the value of these biomarkers for SLE diagnosis and assessment of disease activity.

methodsBlood samples from 58 SLE patients, and 60 healthy controls (HCs) were used for detection of lncRNAs-Cox2 and HOTAIR expression levels by real-time polymerase chain reaction. Both IL-6 and MMP-9 serum levels were assayed by enzyme-linked immunosorbent assay. Lupus activity was assessed with the Systemic Lupus Erythematosus Disease Activity Index (SLEDAI).

resultsThe serum expression levels of lncRNA-Cox2 and HOTAIR were significantly up-regulated in SLE patients vs HCs (fold change [median (IQR) was 1.29(0.81-1.71, P<0.0001) and 2.68(0.95-3.67), P = 0.038) for lncRNA-Cox2 and HOTAIR, respectively. Serum levels of both IL-6 and MMP-9 were significantly high in SLE patients compared with HCs (P≤0.001 for each). The up-regulated lncRNA-Cox2 was positively associated with the presence of neurological manifestations in SLE patients (P = 0.007). Furthermore, HOTAIR expression level had significantly positive correlation with IL-6 (r = 0.578, P<0.0001), MMP-9 level (r = 0.762, P<0.0001), nephritis grades (r = 0.296, P = 0.024) and proteinuria (r = 0.287, P = 0.035). LncRNA-Cox2 showed sensitivity and specificity 72.4%, and 100.0% respectively. HOTAIR sensitivity was 60.3%, and specificity was 100.0%. By multiple logistic regression analysis, lncRNA-Cox2 and HOTAIR were found as SLE independent predictors.

conclusionLncRNA-COX2 and HOTAIR can be used as new non-invasive biomarkers for the diagnosis of SLE.

Indexed as

Lupus Erythematosus, SystemicLupus NephritisRNA, Long NoncodingBiomarkersHumansInterleukin-6Matrix Metalloproteinase 9BiomarkersHOTAIR long untranslated RNA, humanInterleukin-6Matrix Metalloproteinase 9RNA, Long Noncoding

Identifiers

PMID35972968
PMCPMC9380942
OpenAlexW4291994002

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.