ArticleNature biomedical engineering2023
A one-photon endoscope for simultaneous patterned optogenetic stimulation and calcium imaging in freely behaving mice.
Article in Nature biomedical engineering, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Spiral scan and cylindrical deconvolution to maximize image volume and contrast of multiphoton GRIN microendoscopy.Biomedical optics express · 2026Article
- Functional Ultrasound Localization Microscopy on Freely Moving Rats.Research square · 2026Article
- STIMscope: centimeter-scale all-optical imaging and patterned optogenetic manipulation at single-cell resolution.bioRxiv : the preprint server for biology · 2026Article
- 2P-FENDO-II: A fiber bundle microscope for all-optical, large field-of-view brain studies in freely moving mice.Cell reports methods · 2026Article
- Laser-engineered PRIME fiber for panoramic reconfigurable control of neural activity.Nature neuroscience · 2026Article
- Large field-of-view volumetric deep brain imaging through gradient-index lenses.Nature communications · 2025Article
- Multiphoton Neurophotonics: Recent Advances in Imaging and Manipulating Neuronal Circuits.ACS photonics · 2025Review
- The Ferroptosis-Mitochondrial Axis in Depression: Unraveling the Feedforward Loop of Oxidative Stress, Metabolic Homeostasis Dysregulation, and Neuroinflammation.Antioxidants (Basel, Switzerland) · 2025Review
- A sensitive soma-localized red fluorescent calcium indicator for in vivo imaging of neuronal populations at single-cell resolution.PLoS biology · 2025Article
- Aligning brain and behavior.Current opinion in behavioral sciences · 2025Article
- From microelectrode arrays to all-optical and multimodal neural interfaces: emerging platforms for spatiotemporal interrogation ofFrontiers in synaptic neuroscience · 2025Review
- Implantable silicon neural probes with nanophotonic phased arrays for single-lobe beam steering.Communications engineering · 2024Article
- Opto2P-FCM: A MEMS based miniature two-photon microscope with two-photon patterned optogenetic stimulation.bioRxiv : the preprint server for biology · 2024Article
- Recent advances in light patterned optogenetic photostimulation in freely moving mice.Neurophotonics · 2024Article
- Article
- Computer vision-guided open-source active commutator for neural imaging in freely behaving animals.Neurophotonics · 2024Article
- Updated Toolbox for Assessing Neuronal Network Reconstruction after Cell Therapy.Bioengineering (Basel, Switzerland) · 2024Review
- Geometric transformation adaptive optics (GTAO) for volumetric deep brain imaging through gradient-index lenses.Nature communications · 2024Article
- Review
- Pupil engineering for extended depth-of-field imaging in a fluorescence miniscope.Neurophotonics · 2023Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Optogenetics and calcium imaging can be combined to simultaneously stimulate and record neural activity in vivo. However, this usually requires two-photon microscopes, which are not portable nor affordable. Here we report the design and implementation of a miniaturized one-photon endoscope for performing simultaneous optogenetic stimulation and calcium imaging. By integrating digital micromirrors, the endoscope makes it possible to activate any neuron of choice within the field of view, and to apply arbitrary spatiotemporal patterns of photostimulation while imaging calcium activity. We used the endoscope to image striatal neurons from either the direct pathway or the indirect pathway in freely moving mice while activating any chosen neuron in the field of view. The endoscope also allows for the selection of neurons based on their relationship with specific animal behaviour, and to recreate the behaviour by mimicking the natural neural activity with photostimulation. The miniaturized endoscope may facilitate the study of how neural activity gives rise to behaviour in freely moving animals.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.