Evidence map›Paper›PMID 35969701›Full record

ArticleJournal of animal science and technology2022

Transcriptome-wide analysis reveals gluten-induced suppression of small intestine development in young chickens.

Darae Kang, Donghyun Shin, Hosung Choe, Doyon Hwang, Andrew Wange Bugenyi, Chong-Sam Na, Hak-Kyo Lee, Jaeyoung Heo, Kwanseob Shim

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of animal science and technology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Darae KangDepartment of Animal Biotechnology, Jeonbuk National University, Jeonju 54896, Korea.ORCID https://orcid.org/0000-0002-1659-4427
Donghyun ShinDepartment of Agricultural Convergence Technology, Jeonbuk National University, Jeonju 54896, Korea.ORCID https://orcid.org/0000-0002-0819-0553
Hosung ChoeDepartment of Animal Biotechnology, Jeonbuk National University, Jeonju 54896, Korea.ORCID https://orcid.org/0000-0002-1973-3568
Doyon HwangInstitute for Animal Products Quality Evaluation, Sejong 339011, Korea.ORCID https://orcid.org/0000-0003-2845-5093
Andrew Wange BugenyiDepartment of Agricultural Convergence Technology, Jeonbuk National University, Jeonju 54896, Korea.ORCID https://orcid.org/0000-0001-9036-8391
Chong-Sam NaDepartment of Animal Biotechnology, Jeonbuk National University, Jeonju 54896, Korea.ORCID https://orcid.org/0000-0002-8979-5633
Hak-Kyo LeeDepartment of Animal Biotechnology, Jeonbuk National University, Jeonju 54896, Korea.ORCID https://orcid.org/0000-0001-5387-4885
Jaeyoung HeoDepartment of Animal Biotechnology, Jeonbuk National University, Jeonju 54896, Korea.ORCID https://orcid.org/0000-0002-9721-8043
Kwanseob ShimDepartment of Animal Biotechnology, Jeonbuk National University, Jeonju 54896, Korea.ORCID https://orcid.org/0000-0002-4996-3700
Jeonbuk National University · KRKorea Institute for Animal Products Quality Evaluation · KRNational Agricultural Research Organisation · UG

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Wheat gluten is an increasingly common ingredient in poultry diets but its impact on the small intestine in chicken is not fully understood. This study aimed to identify effects of high-gluten diets on chicken small intestines and the variation of their associated transcriptional responses by age. A total of 120 broilers (Ross Strain) were used to perform two animal experiments consisting of two gluten inclusion levels (0% or 25%) by bird's age (1 week or 4 weeks). Transcriptomics and histochemical techniques were employed to study the effect of gluten on their duodenal mucosa using randomly selected 12 broilers (3 chicks per group). A reduction in feed intake and body weight gain was found in the broilers fed a high-gluten containing diet at both ages. Histochemical photomicrographs showed a reduced villus height to crypt depth ratio in the duodenum of gluten-fed broilers at 1 week. We found mainly a significant effect on the gene expression of duodenal mucosa in gluten-fed broilers at 1 week (289 differentially expressed genes [DEGs]). Pathway analyses revealed that the significant DEGs were mainly involved in ribosome, oxidative phosphorylation, and peroxisome proliferator-activated receptor (PPAR) signaling pathways. These pathways are involved in ribosome protein biogenesis, oxidative phosphorylation and fatty acid metabolism, respectively. Our results suggest a pattern of differential gene expression in these pathways that can be linked to chronic inflammation, suppression of cell proliferation, cell cycle arrest and apoptosis. And via such a mode of action, high-gluten inclusion levels in poultry diets could lead to the observed retardation of villi development in the duodenal mucosa of young broiler chicken.

Indexed as

ChickenGlutenRNA sequencingSmall intestinesTranscriptome

Identifiers

PMID35969701
PMCPMC9353357
OpenAlexW4289732286

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.