ArticleeLife2022
Transcriptional heterogeneity and cell cycle regulation as central determinants of Primitive Endoderm priming.
Article in eLife, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 27 citations in OpenAlex.
- Beyond the mean: genetic control of gene expression fidelity and dispersion.bioRxiv : the preprint server for biology · 2026Article
- Post-replicative chromatin accessibility predicts cell fate change.Stem cell reports · 2026Article
- Cell cycle criticality as a mechanism for robust cell population control.Molecular systems biology · 2026Article
- Mitigating Cell Cycle Effects in Multi-Omics Data: Solutions and Analytical Frameworks.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Divergent destinies: insights into the molecular mechanisms underlying EPI and PE fate determination.Life science alliance · 2025Review
- Deep learning-based models for preimplantation mouse and human embryos based on single-cell RNA sequencing.Nature methods · 2025Article
- nf-core/marsseq: systematic preprocessing pipeline for MARS-seq experiments.Bioinformatics advances · 2025Article
- Cell cycle expression heterogeneity predicts degree of differentiation.Briefings in bioinformatics · 2024Article
- Cell cycle expression heterogeneity predicts degree of differentiation.bioRxiv : the preprint server for biology · 2024Article
- Transcription factor co-expression mediates lineage priming for embryonic and extra-embryonic differentiation.Stem cell reports · 2024Article
- Fluorescent biosensors illuminate the spatial regulation of cell signaling across scales.The Biochemical journal · 2023Review
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Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During embryonic development cells acquire identity as they proliferate, implying that an intrinsic facet of cell fate choice requires coupling lineage decisions to cell division. How is the cell cycle regulated to promote or suppress heterogeneity and differentiation? We explore this question combining time lapse imaging with single-cell RNA-seq in the contexts of self-renewal, priming, and differentiation of mouse embryonic stem cells (ESCs) towards the Primitive Endoderm (PrE) lineage. Since ESCs are derived from the inner cell mass (ICM) of the mammalian blastocyst, ESCs in standard culture conditions are transcriptionally heterogeneous containing dynamically interconverting subfractions primed for either of the two ICM lineages, Epiblast and PrE. Here, we find that differential regulation of cell cycle can tip the balance between these primed populations, such that naïve ESC culture promotes Epiblast-like expansion and PrE differentiation stimulates the selective survival and proliferation of PrE-primed cells. In endoderm differentiation, this change is accompanied by a counter-intuitive increase in G1 length, also observed
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.