Hydrolysis capacity of different sized granules in a full-scale aerobic granular sludge (AGS) reactor.

Sara Toja Ortega, Lenno van den Berg, Mario Pronk, Merle K de Kreuk

Abstract read

In one paragraph

Article in Water research X, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed

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1 citing paper in PubMed.

Elucidating the complex hydrolysis and conversion network of xanthan-like extracellular heteropolysaccharides in waste activated sludge fermentation.Water research X · 2025
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5 · Who and what money

Authors and funding

4 authors.

Sara Toja OrtegaSection Sanitary Engineering, Department of Water Management, Delft University of Technology, Stevinweg 1, Delft 2628CN, the Netherlands.

Lenno van den BergSection Sanitary Engineering, Department of Water Management, Delft University of Technology, Stevinweg 1, Delft 2628CN, the Netherlands.

Mario PronkDepartment of Biotechnology, Delft University of Technology, Van der Maasweg 9, Delft, HZ 2629, the Netherlands.

Merle K de KreukSection Sanitary Engineering, Department of Water Management, Delft University of Technology, Stevinweg 1, Delft 2628CN, the Netherlands.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In aerobic granular sludge (AGS) reactors, granules of different sizes coexist in a single reactor. Their differences in settling behaviour cause stratification in the settled granule bed. In combination with substrate concentration gradients over the reactor height during the anaerobic plug-flow feeding regime, this can result in functional differences between granule sizes. In this study, we compared the hydrolytic activity in granules of 4 size ranges (between 0.5 and 4.8 mm diameter) collected from a full-scale AGS installation. Protease and amylase activities were quantified through fluorescent activity assays. To visualise where the hydrolytic active zones were located within the granules, the hydrolysis sites were visualized microscopically after incubating intact and sliced granules with fluorescent casein and starch. The microbial community was studied using fluorescent

Indexed as

Activity stainingAerobic granular sludgeAGS, aerobic granular sludgeAS, activated sludgeBiomass segregationCOD, chemical oxygen demandEBPR, enhanced biological phosphorus removalEPS, extracellular polymeric substancesFISH, fluorescence in situ hybridizationGAO, glycogen-accumulating organismHydrolysisPAO, polyphosphate-accumulating organismPolymeric substratesSBR, sequencing batch reactorSND, simultaneous nitrification-denitrificationSRT, solids retention timeTSS, total suspended solidsVFA, volatile fatty acidVSS, volatile suspended solidsWastewater treatmentWWTP, wastewater treatment plant

Identifiers

PMID35965888

DOI10.1016/j.wroa.2022.100151

PMCPMC9364025

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.