ArticleAnimal microbiome2022
Does swab type matter? Comparing methods for Mannheimia haemolytica recovery and upper respiratory microbiome characterization in feedlot cattle.
Article in Animal microbiome, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 19 citations in OpenAlex.
- Effect of bovine respiratory disease on the respiratory microbiome: a meta-analysis.Frontiers in cellular and infection microbiology · 2023Pooled it
- Optimized approaches to profile low biomass DNA and RNA virome in the upper respiratory tract of beef cattle.Animal microbiome · 2026Article
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- Exploring clonality ofMicrobiology spectrum · 2026Article
- Comparison of short nasal swab and deep nasopharyngeal swab sampling methods to describe BRD-associated viruses and bacteria detected using a metagenomics approach optimized for virus recovery in fall-placed beef calves shortly after feedlot arrival.Veterinary and animal science · 2026Article
- Isolation, Pathogenicity and Genomic Analysis ofMicroorganisms · 2025Article
- Retrospective analysis of antimicrobial resistance associated with bovine respiratory disease.Applied and environmental microbiology · 2025Article
- Aggregate Sampling to Detect Pathogens and Antimicrobial Resistance Genes Associated with Bovine Respiratory Disease in US Feedlots: A Pilot Study.Veterinary sciences · 2025Article
- Article
- Upper respiratory tract detection of Mycoplasma ovipneumoniae employing nasopharyngeal swabs.BMC veterinary research · 2024Article
- Agreement among deep nasopharyngeal sampling culture results for 3 different swab types in preweaning dairy calves.JDS communications · 2024Article
- Effects of vaccination and interventions on nasal microbiome and BRD-associated pathogens in calves.Frontiers in microbiology · 2024Article
- Article
- Tulathromycin metaphylaxis increases nasopharyngeal isolation of multidrug resistantFrontiers in veterinary science · 2023Article
- Effects of respiratory virus vaccination and bovine respiratory disease on the respiratory microbiome of feedlot cattle.Frontiers in microbiology · 2023Article
- Article
- Thoracic Ultrasonography Findings and Their Association With Respiratory Pathogens in 221 Young Beef Cattle at Fattening Farms: A Cross-Sectional Study.Journal of veterinary internal medicineArticle
Corrections and comments
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Authors and funding
11 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBovine respiratory disease (BRD) is caused by interactions among host, environment, and pathogens. One standard method for antemortem pathogen identification in cattle with BRD is deep-guarded nasopharyngeal swabbing, which is challenging, costly, and waste generating. The objective was to compare the ability to recover Mannheimia haemolytica and compare microbial community structure using 29.5 inch (74.9 cm) deep-guarded nasopharyngeal swabs, 16 inch (40.6 cm) unguarded proctology swabs, or 6 inch (15.2 cm) unguarded nasal swabs when characterized using culture, real time-qPCR, and 16S rRNA gene sequencing. Samples for aerobic culture, qPCR, and 16S rRNA gene sequencing were collected from the upper respiratory tract of cattle 2 weeks after feedlot arrival.
resultsThere was high concordance of culture and qPCR results for all swab types (results for 77% and 81% of sampled animals completely across all 3 swab types for culture and qPCR respectively). Microbial communities were highly similar among samples collected with different swab types, and differences identified relative to treatment for BRD were also similar. Positive qPCR results for M. haemolytica were highly concordant (81% agreed completely), but samples collected by deep-guarded swabbing had lower amounts of Mh DNA identified (Kruskal-Wallis analysis of variance on ranks, P < 0.05; Dunn-test for pairwise comparison with Benjamini-Hochberg correction, P < 0.05) and lower frequency of positive compared to nasal and proctology swabs (McNemar's Chi-square test, P < 0.05).
conclusionsThough differences existed among different types of swabs collected from individual cattle, nasal swabs and proctology swabs offer comparable results to deep-guarded nasopharyngeal swabs when identifying and characterizing M. haemolytica by culture, 16S rRNA gene sequencing, and qPCR.
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