Evidence map›Paper›PMID 35962914›Full record

ArticleBiometals : an international journal on the role of metal ions in biology, biochemistry, and medicine2022

Caught red handed: modeling and confirmation of the myeloperoxidase ceruloplasmin alpha-thrombin complex.

Yana A Zabrodskaya, Vladimir V Egorov, Alexey V Sokolov, Alexey V Shvetsov, Yulia E Gorshkova, Oleksandr I Ivankov, Valeria A Kostevich, Nikolay P Gorbunov, Edward S Ramsay, Natalya D Fedorova and 2 more

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In one paragraph

Article in Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 7 institutions in 1 country.

Yana A ZabrodskayaSmorodintsev Research Institute of Influenza, Russian Ministry of Health, 15/17 Ulitsa Prof. Popova, St. Petersburg, Russia, 197376. yana@zabrodskaya.net.ORCID 0000-0003-2012-9461
Vladimir V EgorovSmorodintsev Research Institute of Influenza, Russian Ministry of Health, 15/17 Ulitsa Prof. Popova, St. Petersburg, Russia, 197376.
Alexey V SokolovInstitute of Experimental Medicine, 12 Ulitsa Akademika Pavlova, St. Petersburg, Russia, 197376.
Alexey V ShvetsovPeter the Great Saint Petersburg Polytechnic University, 29 Ulitsa Polytechnicheskaya, St. Petersburg, Russia, 194064.
Yulia E GorshkovaInternational Intergovernmental Organization Joint Institute for Nuclear Research, 6 Ulitsa Joliot-Curie, Dubna, Russia, 141980.
Oleksandr I IvankovInternational Intergovernmental Organization Joint Institute for Nuclear Research, 6 Ulitsa Joliot-Curie, Dubna, Russia, 141980.
Valeria A KostevichInstitute of Experimental Medicine, 12 Ulitsa Akademika Pavlova, St. Petersburg, Russia, 197376.
Nikolay P GorbunovInstitute of Experimental Medicine, 12 Ulitsa Akademika Pavlova, St. Petersburg, Russia, 197376.
Edward S RamsaySaint Petersburg Pasteur Institute, 14 Ulitsa Mira, St. Petersburg, Russia, 197101.
Natalya D FedorovaPetersburg Nuclear Physics Institute Named by B. P. Konstantinov of National Research Center, Kurchatov Institute, 1 mkr. Orlova roshcha, Gatchina, Russia, 188300.
Andrey B BondarenkoSmorodintsev Research Institute of Influenza, Russian Ministry of Health, 15/17 Ulitsa Prof. Popova, St. Petersburg, Russia, 197376.
Vadim B VasilyevInstitute of Experimental Medicine, 12 Ulitsa Akademika Pavlova, St. Petersburg, Russia, 197376.
Institute of Experimental Medicine · RUMinistry of Health of the Russian Federation · RUPeter the Great St. Petersburg Polytechnic University · RUJoint Institute for Nuclear Research · RUKazan Federal University · RUPetersburg Nuclear Physics Institute · RUSaint Petersburg Pasteur Institute · RU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The work is devoted to the study of the structural characteristics of the myeloperoxidase-ceruloplasmin-thrombin complex using small-angle neutron scattering methods in combination with computer modeling, as well as surface plasmon resonance and solid-phase enzyme assay. We have previously shown that the functioning of active myeloperoxidase during inflammation, despite the presence in the blood of an excess of ceruloplasmin which inhibits its activity, is possible due to the partial proteolysis of ceruloplasmin by thrombin. In this study, the myeloperoxidase-ceruloplasmin-thrombin heterohexamer was obtained in vitro. The building of a heterohexamer full-atomic model in silico, considering the glycosylation of the constituent proteins, confirmed the absence of steric barriers for the formation of protein-protein contacts. It was shown that the partial proteolysis of ceruloplasmin does not affect its ability to bind to myeloperoxidase, and a structural model of the heterohexamer was obtained using the small-angle neutron scattering method.

Indexed as

CeruloplasminPeroxidaseColoring AgentsEnzyme AssaysThrombinCeruloplasminColoring AgentsPeroxidaseThrombinCeruloplasminHalogenative stressInflammationMolecular modelingMyeloperoxidaseReactive halogen speciesSmall-angle neutron scatteringThrombin

Identifiers

PMID35962914
PMCPMC9375587
OpenAlexW4291209146

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.