ArticlePeerJ2022
Skimming for barcodes: rapid production of mitochondrial genome and nuclear ribosomal repeat reference markers through shallow shotgun sequencing.
Article in PeerJ, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Surveying Biodiverse Coral Reef Ecosystems Across the Tree of Life With Environmental DNA.Molecular ecology resources · 2026Article
- Environmental filtering shapes patch dynamics across isolated mesophotic reefs.Science advances · 2026Article
- A Prickly Problem: Genome Skimming Reveals Varying Levels of Phylogenetic Diversity in the Freshwater CrayfishEcology and evolution · 2026Article
- Accounting for Intra- and Intergenomic Sequence Variation in Reference Barcodes Improves eDNA Metabarcoding Biodiversity Assessment.Molecular ecology resources · 2026Article
- Validating COI eDNA Metabarcoding Primers for Detection of Subterranean Fauna.Molecular ecology resources · 2026Article
- Article
- Revised diagnosis of the flathead genusZooKeys · 2026Article
- DNA barcoding of Austrian terrestrial isopods (Crustacea: Isopoda: Oniscidea) reveals potential cryptic diversity.PloS one · 2026Article
- Analysis of the Entire Mitogenome of the Threatened Freshwater StingrayInternational journal of molecular sciences · 2025Article
- Mitogenomes of mosquito species of Harris County in Texas.Scientific reports · 2025Article
- Phylogenetic taxonomy of the Zambian Anopheles coustani group using a mitogenomics approach.Malaria journal · 2025Article
- Article
- Article
- Advancing the environmental DNA and RNA toolkit for aquatic ecosystem monitoring and management.PeerJ · 2025Article
- Endemic Radiation of African Moonfish,Biomolecules · 2024Article
- Skimming genomes for systematics and DNA barcodes of corals.Ecology and evolution · 2024Article
- Article
- Mitogenomic Characterization and Phylogenetic Placement of African Hind,International journal of molecular sciences · 2024Article
- The first complete mitochondrional genome ofF1000Research · 2024Article
- Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA barcoding is critical to conservation and biodiversity research, yet public reference databases are incomplete. Existing barcode databases are biased toward cytochrome oxidase subunit I (COI) and frequently lack associated voucher specimens or geospatial metadata, which can hinder reliable species assignments. The emergence of metabarcoding approaches such as environmental DNA (eDNA) has necessitated multiple marker techniques combined with barcode reference databases backed by voucher specimens. Reference barcodes have traditionally been generated by Sanger sequencing, however sequencing multiple markers is costly for large numbers of specimens, requires multiple separate PCR reactions, and limits resulting sequences to targeted regions. High-throughput sequencing techniques such as genome skimming enable assembly of complete mitogenomes, which contain the most commonly used barcoding loci (
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