Evidence map›Paper›PMID 35955668›Full record

ArticleInternational journal of molecular sciences2022

Quantitative Characterisation of Low Abundant Yeast Mitochondrial Proteins Reveals Compensation for Haplo-Insufficiency in Different Environments.

Alkisti Manousaki, James Bagnall, David Spiller, Laura Natalia Balarezo-Cisneros, Michael White, Daniela Delneri

Open access · goldAbstract read
In one paragraph

Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.1field-weighted citation impact, top 57% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Alkisti ManousakiManchester Institute of Biotechnology, Faculty of Biology, Medicine and Health, The University of Manchester, 131 Princess Street, Manchester M1 7DN, UK.
James BagnallDivision of Diabetes, Endocrinology and Gastroenterology Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, The University of Manchester, Oxford Road, Manchester M13 9PT, UK.
David SpillerPlatform Sciences, Enabling Technologies & Infrastructure, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, The University of Manchester, Oxford Road, Manchester M13 9PT, UK.ORCID 0000-0003-2502-6787
Laura Natalia Balarezo-CisnerosManchester Institute of Biotechnology, Faculty of Biology, Medicine and Health, The University of Manchester, 131 Princess Street, Manchester M1 7DN, UK.
Michael WhiteDivision of Molecular and Cellular Function, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, The University of Manchester, Oxford Road, Manchester M13 9PT, UK.
Daniela DelneriManchester Institute of Biotechnology, Faculty of Biology, Medicine and Health, The University of Manchester, 131 Princess Street, Manchester M1 7DN, UK.ORCID 0000-0001-8070-411X
Manchester Academic Health Science Centre · GBUniversity of Manchester · GB

Funding

Biotechnology and Biological Sciences Research Council BB/K003097/1Biotechnology and Biological Sciences Research Council BB/M011208/1Biotechnology and Biological Sciences Research Council BB/T002123/1
6 · The paper itself

Abstract

The quantification of low abundant membrane-binding proteins such as transcriptional factors and chaperones has proven difficult, even with the most sophisticated analytical technologies. Here, we exploit and optimise the non-invasive Fluorescence Correlation Spectroscopy (FCS) for the quantitation of low abundance proteins, and as proof of principle, we choose two interacting proteins involved in the fission of mitochondria in yeast, Fis1p and Mdv1p. In

Indexed as

Saccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsAdaptor Proteins, Signal TransducingGTP PhosphohydrolasesMembrane ProteinsMitochondriaMitochondrial ProteinsAdaptor Proteins, Signal TransducingGTP PhosphohydrolasesMembrane ProteinsMitochondrial ProteinsSaccharomyces cerevisiae ProteinsFCShaplo-insufficiencymitochondrial proteinsprotein quantificationyeast

Identifiers

PMID35955668
PMCPMC9369417
OpenAlexW4289335916

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.