ArticleInternational journal of molecular sciences2022
BPA Decreases PDCD4 in Bovine Granulosa Cells Independently of miR-21 Inhibition.
Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 19 citations in OpenAlex.
- Altered miRNA Expression Due to Bisphenol A Exposure and Associated Health Implications: A Narrative Review.Journal of xenobiotics · 2026Review
- An Overview of How Epigenetics, MicroRNA-21, and Endocrine Disrupting Compounds Affect Oocyte Maturation and Pre-Implantation Embryo Development.Journal of developmental biology · 2026Review
- Regulatory mechanisms of microRNAs in reproductive toxicity induced by environmental pollutants: a mini review.Archives of toxicology · 2026Review
- Transgenerational reproductive risks of BPA: epigenetic mechanisms and biomarker applications. A critical review.Environmental epigenetics · 2026Review
- miR-361-5p regulates SLC25A24 to maintain mitochondrial function and alleviate granulosa cell dysfunction in diminished ovarian reserve.Journal of assisted reproduction and genetics · 2025Article
- Extracellular vesicles: key mediators inFrontiers in veterinary science · 2025Review
- miRNA profiling of chicken follicles during follicular development.Scientific reports · 2024Article
- miR-423 sponged by lncRNA NORHA inhibits granulosa cell apoptosis.Journal of animal science and biotechnology · 2023Article
- Synthetic miR-21 decoy circularized by tRNA splicing mechanism inhibited tumorigenesis in glioblastomaMolecular therapy. Nucleic acids · 2023Article
- Special Issue "Molecular Mechanisms of Bisphenol A Toxicity and Effects of Environmental Levels on Health".International journal of molecular sciences · 2023Article
- The relationship between miR-21, DNA methylation, and bisphenol a in bovine COCs and granulosa cells.Frontiers in cell and developmental biology · 2023Article
- Bisphenols A and F, but not S, induce apoptosis in bovine granulosa cellsFrontiers in endocrinology · 2022Article
- A focused evaluation of genetic and epigenetic contributions to common infertility conditions.Therapeutic advances in reproductive healthReview
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
microRNAs (miRNAs) are susceptible to environmental factors that might affect cellular function and impose negative effects on female reproduction. miR-21 is the most abundant miRNA in bovine granulosa cells and is widely reported as affected by Bisphenol A (BPA) exposure, yet the cause and consequences are not entirely elucidated. BPA is a synthetic endocrine disruptor associated with poor fertility. miR-21 function in bovine granulosa cells is investigated utilizing locked nucleic acid (LNA) oligonucleotides to suppress miR-21. Before measuring apoptosis and quantifying miR-21 apoptotic targets PDCD4 and PTEN, transfection was optimized and validated. BPA was introduced to see how it affects miR-21 regulation and which BPA-mediated effects are influenced by miR-21. miR-21 knockdown and specificity against additional miRNAs were confirmed. miR-21 was found to have antiapoptotic effects, which could be explained by its effect on the proapoptotic target PDCD4, but not PTEN. Previous findings of miR-21 overexpression were validated using BPA treatments, and the temporal influence of BPA on miR-21 levels was addressed. Finally, BPA effects on upstream regulators, such as VMP1 and STAT3, explain the BPA-dependent upregulation of miR-21 expression. Overall, this research enhances our understanding of miR-21 function in granulosa cells and the mechanisms of BPA-induced reproductive impairment.
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