Evidence map›Paper›PMID 35953779›Full record

ArticleBMC genomics2022

Effects of berberine hydrochloride on immune response in the crab Charybdis japonica.

Tian-Heng Gao, Ming-Ming Han, Hui Zhou, Chen-Xi Zhu, Ying Yang, Zakaria Zuraini, Yan-Xia Guo, Qi-Chen Jiang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.8field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 2 countries.

Tian-Heng GaoInstitute of Marine Biology, College of Oceanography, Hohai University, Nanjing, 210024, China.
Ming-Ming HanBiology Program, School of Distance Education, Universiti Sains Malaysia, 11800, Minden, Penang, Malaysia.
Hui ZhouJiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, 210023, China.
Chen-Xi ZhuFreshwater Fisheries Research Institute of Jiangsu Province, 79 Chating East Street, Nanjing, 210017, China.
Ying YangFreshwater Fisheries Research Institute of Jiangsu Province, 79 Chating East Street, Nanjing, 210017, China.
Zakaria ZurainiBiology Program, School of Distance Education, Universiti Sains Malaysia, 11800, Minden, Penang, Malaysia.
Yan-Xia GuoJiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, 210023, China.
Qi-Chen JiangFreshwater Fisheries Research Institute of Jiangsu Province, 79 Chating East Street, Nanjing, 210017, China. qichenjiang@live.cn.
Shandong Freshwater Fisheries Research Institute · CNNanjing Normal University · CNUniversiti Sains Malaysia · MYHohai University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Berberine hydrochloride is the main effective component of Coptis spp. used in Chinese herbal medicine and its underlying molecular mechanisms, responsible for inducing effects in crustacean species, are not fully understood. In this study, the molecular response of the crab Charybdis japonica to berberine hydrochloride exposure was studied using transcriptome sequencing. The survival rate, gene expression and activities of several immune enzymes were measured after berberine hydrochloride treatments, with or without injection of the pathogenic bacterium Aeromonas hydrophila. A total of 962 differentially expressed genes (464 up-regulated and 498 down-regulated) were observed during exposure to 100 mg/L of berberine hydrochloride and in the control group after 48 h. Enrichment analysis revealed that these genes are involved in metabolism, cellular processes, signal transduction and immune functions, indicating that exposure to berberine hydrochloride activated the immune complement system. This bioactive compound simultaneously activated fibrinogen beta (FGB), fibrinogen alpha (FGA), alpha-2-macroglobulin (A2M), kininogen (KNG), fibrinogen gamma chain (FGB), alpha-2-HS-glycoprotein (AHSG), caspase-8 (CASP8), cathepsin L (CTSL), adenylate cyclase 3 (Adcy3) and MMP1. Its action could significantly increase the survival rate of the crabs injected with A. hydrophila and promote the activity of LZM, Caspas8, FGA, ACP and AKP in the hepatopancreas. When A. hydrophila was added, the neutralization of 300 mg/L berberine hydrochloride maximized the activities of Caspas8, LZM, ACP and AKP. Our results provide a new understanding of the potential effects of berberine hydrochloride on the immune system mechanisms in crustaceans.

Indexed as

BerberineBrachyuraAnimalsFibrinogenHepatopancreasImmunityBerberineFibrinogenberberine hydrochlorideCharybdis japonicaImmune responseTranscriptome sequencing

Identifiers

PMID35953779
PMCPMC9373360
OpenAlexW4291000324

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.