Evidence map›Paper›PMID 35953650›Full record

ArticleNature biomedical engineering2022

Rapid detection of SARS-CoV-2 RNA in saliva via Cas13.

Sita S Chandrasekaran, Shreeya Agrawal, Alison Fanton, Aditya R Jangid, Bérénice Charrez, Arturo M Escajeda, Sungmin Son, Roger Mcintosh, Huyen Tran, Abdul Bhuiya and 27 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature biomedical engineering, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
58citing papers in PubMed, 1 pooled it
11.7field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

58 citing papers in PubMed, 1 synthesis or guideline pooled it, 124 citations in OpenAlex.

  1. Pooled it
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  9. Targeting extrachromosomal DNA in human cancers.Nature reviews. Drug discovery · 2026
    Review
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

37 authors at 9 institutions in 2 countries.

Sita S Chandrasekaran *Department of Bioengineering, University of California, Berkeley, Berkeley, CA, USA.
Shreeya Agrawal *Department of Bioengineering, University of California, Berkeley, Berkeley, CA, USA.
Alison Fanton *Department of Bioengineering, University of California, Berkeley, Berkeley, CA, USA.
Aditya R Jangid *Department of Bioengineering, University of California, Berkeley, Berkeley, CA, USA.
Bérénice CharrezUniversity of California, Berkeley-University of California, San Francisco Graduate Program in Bioengineering, Berkeley, CA, USA.
Arturo M EscajedaWainamics Inc., Pleasanton, CA, USA.
Sungmin SonDepartment of Bioengineering, University of California, Berkeley, Berkeley, CA, USA.
Roger McintoshWainamics Inc., Pleasanton, CA, USA.
Huyen TranWainamics Inc., Pleasanton, CA, USA.
Abdul BhuiyaDepartment of Bioengineering, University of California, Berkeley, Berkeley, CA, USA.
María Díaz de León DerbyDepartment of Bioengineering, University of California, Berkeley, Berkeley, CA, USA.
Neil A SwitzDepartment of Physics and Astronomy, San José State University, San José, CA, USA.
Maxim ArmstrongMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
Andrew R HarrisDepartment of Bioengineering, University of California, Berkeley, Berkeley, CA, USA.
Noam PrywesInnovative Genomics Institute, University of California, Berkeley, Berkeley, CA, USA.
Maria LukarskaInnovative Genomics Institute, University of California, Berkeley, Berkeley, CA, USA.
Scott B BieringDivision of Infectious Diseases and Vaccinology, School of Public Health, University of California, Berkeley, Berkeley, CA, USA.
Dylan C J SmockInnovative Genomics Institute, University of California, Berkeley, Berkeley, CA, USA.
Amanda MokCenter for Computational Biology, University of California, Berkeley, Berkeley, CA, USA.
Gavin J KnottInnovative Genomics Institute, University of California, Berkeley, Berkeley, CA, USA.
Qi DangInnovative Genomics Institute, University of California, Berkeley, Berkeley, CA, USA.
Erik Van DisDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA.
Eli DuganInnovative Genomics Institute, University of California, Berkeley, Berkeley, CA, USA.
Shin KimInnovative Genomics Institute, University of California, Berkeley, Berkeley, CA, USA.
Tina Y LiuInnovative Genomics Institute, University of California, Berkeley, Berkeley, CA, USA.
IGI Testing Consortium
Erica A MoehleInnovative Genomics Institute, University of California, Berkeley, Berkeley, CA, USA.
Katherine KogutCenter for Environmental Research and Community Health (CERCH), School of Public Health, University of California, Berkeley, Berkeley, CA, USA.
Brenda EskenaziCenter for Environmental Research and Community Health (CERCH), School of Public Health, University of California, Berkeley, Berkeley, CA, USA.
Eva HarrisDivision of Infectious Diseases and Vaccinology, School of Public Health, University of California, Berkeley, Berkeley, CA, USA.
Sarah A StanleyDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA.
Liana F LareauDepartment of Bioengineering, University of California, Berkeley, Berkeley, CA, USA.
Ming X TanWainamics Inc., Pleasanton, CA, USA.
Daniel A FletcherDepartment of Bioengineering, University of California, Berkeley, Berkeley, CA, USA.
Jennifer A DoudnaInnovative Genomics Institute, University of California, Berkeley, Berkeley, CA, USA. doudna@berkeley.edu.
David F SavageDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA. savage@berkeley.edu.
Patrick D HsuDepartment of Bioengineering, University of California, Berkeley, Berkeley, CA, USA. pdhsu@berkeley.edu.ORCID http://orcid.org/0000-0002-9380-2648
Innovative Genomics Institute · USUniversity of California, Berkeley · USCenter for Environmental Health · USAustralian Regenerative Medicine Institute · AUBerkeley Public Health Division · USGladstone Institutes · USLawrence Berkeley National Laboratory · USSan Jose State University · USUniversity of California, San Francisco · US

Funding

Eukaryotic transcriptome engineering via sequence-specific regulation of endogenous RNADP5OD021369 · OD · UNIVERSITY OF CALIFORNIA BERKELEY · PI HSU, PATRICK · 2015 to 2018
$2.4M
Molecular tools for targeting RNAR01GM131073 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI HSU, PATRICK · 2019 to 2022
$1.3M
Engineering CRISPR-Cas proteins for conditional and robust interrogation of the genomeR01GM127463 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI SAVAGE, DAVID FRANK · 2019 to 2022
$1.2M
Mechanistic studies of RNA-targeting CRISPR systemsR01GM132465 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI HSU, PATRICK, LYUMKIS, DMITRY · 2020 to 2022
$908k
Howard Hughes Medical InstituteNIGMS NIH HHS R01 GM127463NIGMS NIH HHS R01 GM131073NIGMS NIH HHS R01 GM132465NIH HHS DP5 OD021369
6 · The paper itself

Abstract

Rapid nucleic acid testing is central to infectious disease surveillance. Here, we report an assay for rapid COVID-19 testing and its implementation in a prototype microfluidic device. The assay, which we named DISCoVER (for diagnostics with coronavirus enzymatic reporting), involves extraction-free sample lysis via shelf-stable and low-cost reagents, multiplexed isothermal RNA amplification followed by T7 transcription, and Cas13-mediated cleavage of a quenched fluorophore. The device consists of a single-use gravity-driven microfluidic cartridge inserted into a compact instrument for automated running of the assay and readout of fluorescence within 60 min. DISCoVER can detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in saliva with a sensitivity of 40 copies μl

Indexed as

COVID-19SARS-CoV-2COVID-19 TestingHumansRNA, ViralSalivaRNA, Viral

Identifiers

PMID35953650
PMCPMC10367768
OpenAlexW4292330674

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.