ArticleNature communications2022
Megahertz pulse trains enable multi-hit serial femtosecond crystallography experiments at X-ray free electron lasers.
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Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 14 citations in OpenAlex.
- Lysozyme revisited: evaluating models of a reference protein in structural biology.Current research in structural biology · 2026Article
- Wavefront sensing at a high-repetition-rate X-ray free-electron laser.Journal of synchrotron radiation · 2026Article
- Sample delivery methods for protein X-ray crystallography with a special focus on sample consumption.Nature communications · 2025Review
- Damage before destruction? X-ray-induced changes in single-pulse serial femtosecond crystallography.IUCrJ · 2025Article
- Supercritical density fluctuations and structural heterogeneity in supercooled water-glycerol microdroplets.Nature communications · 2024Article
- Structural biology in the age of X-ray free-electron lasers and exascale computing.Current opinion in structural biology · 2024Review
- A comparative review of time-resolved x-ray and electron scattering to probe structural dynamics.Structural dynamics (Melville, N.Y.) · 2024Review
- Kilohertz droplet-on-demand serial femtosecond crystallography at the European XFEL station FXE.Structural dynamics (Melville, N.Y.) · 2024Article
- A phenomenological model of the X-ray pulse statistics of a high-repetition-rate X-ray free-electron laser.IUCrJ · 2023Article
- Electrically stimulated droplet injector for reduced sample consumption in serial crystallography.Biophysical reports · 2022Article
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Authors and funding
68 authors at 20 institutions in 8 countries.
Susannah HolmesDepartment of Mathematical and Physical Sciences, School of Engineering, Computing and Mathematical Sciences, La Trobe University, Melbourne, VIC, 3086, Australia.
Henry J KirkwoodEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.ORCID 0000-0001-6792-3844
Richard BeanEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
Klaus GiewekemeyerEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
Andrew V MartinSchool of Science, RMIT University, Melbourne, VIC, 3000, Australia.ORCID 0000-0003-3704-1829
Marjan Hadian-JaziDepartment of Mathematical and Physical Sciences, School of Engineering, Computing and Mathematical Sciences, La Trobe University, Melbourne, VIC, 3086, Australia.
Max O WiedornCenter for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany.
Dominik OberthürCenter for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany.
Hugh MarmanDepartment of Mathematical and Physical Sciences, School of Engineering, Computing and Mathematical Sciences, La Trobe University, Melbourne, VIC, 3086, Australia.
Luigi AdrianoDeutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany.
Nasser Al-QudamiEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
Saša BajtCenter for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany.ORCID 0000-0002-7163-7602
Imrich BarákInstitute of Molecular Biology, SAS, Dubravska cesta 21, 845 51, Bratislava, Slovakia.ORCID 0000-0002-3690-404X
Sadia BariDeutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany.ORCID 0000-0003-3985-2051
Johan BieleckiEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
Sandor BrockhauserEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.ORCID 0000-0002-9700-4803
Mathew A ColemanLawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA, 94550, USA.
Francisco Cruz-MazoDept. de Ingeniería Aeroespacial y Mecánica de Fluidos, ETSI, Universidad de Sevilla, 41092, Sevilla, Spain.ORCID 0000-0002-3765-5540
Cyril DanilevskiEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
Katerina DörnerEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
Alfonso M Gañán-CalvoDept. de Ingeniería Aeroespacial y Mecánica de Fluidos, ETSI, Universidad de Sevilla, 41092, Sevilla, Spain.ORCID 0000-0002-7552-6184
Rita GraceffaEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.ORCID 0000-0002-8815-8640
Hans FanghorEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.ORCID 0000-0001-5494-7193
Michael HeymannInstitute of Biomaterials and Biomolecular Systems, University of Stuttgart, Am Pfaffenwaldring 57, 70569, Stuttgart, Germany.ORCID 0000-0002-9278-8207
Matthias FrankLawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA, 94550, USA.ORCID 0000-0002-5244-0791
Alexander KaukherEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
Yoonhee KimEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
Bostjan KobeSchool of Chemistry and Molecular Biosciences, Institute for Molecular Bioscience and Australian Infectious Diseases Research Centre, University of Queensland, Brisbane, QLD, 4072, Australia.ORCID 0000-0001-9413-9166
Juraj KnoškaCenter for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany.ORCID 0000-0001-7258-6256
Torsten LaurusDeutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany.
Romain LetrunEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.ORCID 0000-0002-0569-5193
Luis MaiaEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
Marc MesserschmidtSchool of Molecular Science, Arizona State University, Tempe, AZ, 85281, USA.
Markus MetzCenter for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany.
Thomas MichelatEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.ORCID 0000-0002-5689-8759
Grant MillsEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
Serguei MolodtsovEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
Diana C F MonteiroThe Hamburg Centre for Ultrafast Imaging, Luruper Chaussee 149, Hamburg, 22761, Germany.ORCID 0000-0002-6318-7306
Andrew J MorganCenter for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany.ORCID 0000-0002-9163-4249
Astrid MünnichEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
Gisel E Peña MurilloCenter for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany.ORCID 0000-0001-7842-4987
Gianpietro PrevitaliEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
Adam RoundEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.ORCID 0000-0002-0723-8228
Tokushi SatoEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
Robin SchubertEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.ORCID 0000-0002-6213-2872
Joachim SchulzEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.ORCID 0000-0002-9310-8832
Megan ShelbyLawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA, 94550, USA.
Carolin SeuringCenter for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany.ORCID 0000-0003-1000-0859
Jonas A SellbergBiomedical and X-ray Physics, Department of Applied Physics, AlbaNova University Center, KTH Royal Institute of Technology, SE-106 91, Stockholm, Sweden.ORCID 0000-0003-2793-5052
Marcin SikorskiEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
Alessandro SilenziEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
Stephan SternEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
Jola Sztuk-DambietzEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
Janusz SzubaEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
Martin TrebbinInstitute of Experimental Physics, TU Bergakademie Freiberg, Leipziger, Str. 23, 09599, Freiberg, Germany.ORCID 0000-0001-8278-5865
Patrick VagovicEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
Thomas VeInstitute for Glycomics, Griffith University, Southport, QLD, 4222, Australia.ORCID 0000-0002-0113-1905
Britta WeinhausenEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
Krzysztof WronaEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
Paul Lourdu XavierEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
Chen XuEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
Oleksandr YefanovCenter for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany.
Keith A NugentDepartment of Quantum Science and Technology, Research School of Physics, Australian National University, Canberra, ACT, 2601, Australia.
Henry N ChapmanCenter for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany.ORCID 0000-0002-4655-1743
Adrian P MancusoEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
Anton BartyCenter for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany.
Brian AbbeyDepartment of Mathematical and Physical Sciences, School of Engineering, Computing and Mathematical Sciences, La Trobe University, Melbourne, VIC, 3086, Australia. b.abbey@latrobe.edu.au.ORCID 0000-0001-6504-0503
Connie DarmaninDepartment of Mathematical and Physical Sciences, School of Engineering, Computing and Mathematical Sciences, La Trobe University, Melbourne, VIC, 3086, Australia. c.darmanin@latrobe.edu.au.ORCID 0000-0002-4449-2233
European X-Ray Free-Electron Laser · DEUniversität Hamburg · DELa Trobe University · AUDeutsches Elektronen-Synchrotron DESY · DELawrence Livermore National Laboratory · USAlbaNova · SEArizona State University · USAustralian National University · AUAustralian Nuclear Science and Technology Organisation · AUGriffith University · AUHauptman-Woodward Medical Research Institute · USInstitute of Molecular Biology of the Slovak Academy of Sciences · SKITMO University · RUMax Planck Institute for the Structure and Dynamics of Matter · DEPrinceton University · USRMIT University · AUThe University of Queensland · AUTU Bergakademie Freiberg · DEUniversidad de Sevilla · ESUniversity of Stuttgart · DE
Funding
Technology development for biological imaging with x-ray free electron lasersR01GM117342 · NIGMS · LAWRENCE LIVERMORE NATIONAL SECURITY, LLC · PI FRANK, MATTHIAS · 2016 to 2024
$4.8MNIGMS NIH HHS R01 GM117342
Abstract
The European X-ray Free Electron Laser (XFEL) and Linac Coherent Light Source (LCLS) II are extremely intense sources of X-rays capable of generating Serial Femtosecond Crystallography (SFX) data at megahertz (MHz) repetition rates. Previous work has shown that it is possible to use consecutive X-ray pulses to collect diffraction patterns from individual crystals. Here, we exploit the MHz pulse structure of the European XFEL to obtain two complete datasets from the same lysozyme crystal, first hit and the second hit, before it exits the beam. The two datasets, separated by <1 µs, yield up to 2.1 Å resolution structures. Comparisons between the two structures reveal no indications of radiation damage or significant changes within the active site, consistent with the calculated dose estimates. This demonstrates MHz SFX can be used as a tool for tracking sub-microsecond structural changes in individual single crystals, a technique we refer to as multi-hit SFX.
Indexed as
ElectronsLasersCrystallography, X-RayRadiographyX-Rays
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