ArticleAdvanced healthcare materials2022
The Modular µSiM Reconfigured: Integration of Microfluidic Capabilities to Study In Vitro Barrier Tissue Models under Flow.
Article in Advanced healthcare materials, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed.
- Bacterial extracellular vesicles indirectly destabilize a human stem cell-derived blood-brain barrier on-chip through pro-inflammatory stimulation of immune cells.Lab on a chip · 2026Article
- Modular Integration of Impedance Sensing for Real-Time Assessment of Barrier Integrity.bioRxiv : the preprint server for biology · 2026Article
- Bacterial extracellular vesicles indirectly destabilize a human stem cell-derived blood-brain barrier on-chip through pro-inflammatory stimulation of immune cells.bioRxiv : the preprint server for biology · 2026Article
- Shear Conditioning Promotes Microvascular Endothelial Barrier Resilience in a Human BBB-on-a-Chip Model of Systemic Inflammation Leading to Astrogliosis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Fluid flow impacts endothelial-monocyte interactions in a model of vascular inflammatory fibrosis.Scientific reports · 2025Article
- Sided Stimulation of Endothelial Cells Modulates Neutrophil Trafficking in an In Vitro Sepsis Model.Advanced healthcare materials · 2024Article
- Microengineering 3D Collagen Matrices with Tumor-Mimetic Gradients in Fiber Alignment.Advanced functional materials · 2024Article
- Transforming Static Barrier Tissue Models into Dynamic Microphysiological Systems.Journal of visualized experiments : JoVE · 2024Article
- Use of the MicroSiM (µSiM) Barrier Tissue Platform for Modeling the Blood-Brain Barrier.Journal of visualized experiments : JoVE · 2024Article
- A comprehensive overview of advanced dynamicTissue barriers · 2024Review
- Modeling Brain Vasculature Immune Interactions In Vitro.Cold Spring Harbor perspectives in medicine · 2023Review
- Electropatterning-Contemporary developments for selective particle arrangements employing electrokinetics.Electrophoresis · 2023Review
- A tissue chip with integrated digital immunosensors: In situ brain endothelial barrier cytokine secretion monitoring.Biosensors & bioelectronics · 2023Article
- The critical role of neutrophil-endothelial cell interactions in sepsis: new synergistic approaches employing organ-on-chip, omics, immune cell phenotyping andFrontiers in cellular and infection microbiology · 2023Review
- Microengineering 3D Collagen Hydrogels with Long-Range Fiber Alignment.Journal of visualized experiments : JoVE · 2022Article
- The Modular µSiM: A Mass Produced, Rapidly Assembled, and Reconfigurable Platform for the Study of Barrier Tissue Models In Vitro.Advanced healthcare materials · 2022Article
- A miniaturized 3D printed pressure regulator (µPR) for microfluidic cell culture applications.Scientific reports · 2022Article
- Local extensional flows promote long-range fiber alignment in 3D collagen hydrogels.Biofabrication · 2022Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Microfluidic tissue barrier models have emerged to address the lack of physiological fluid flow in conventional "open-well" Transwell-like devices. However, microfluidic techniques have not achieved widespread usage in bioscience laboratories because they are not fully compatible with traditional experimental protocols. To advance barrier tissue research, there is a need for a platform that combines the key advantages of both conventional open-well and microfluidic systems. Here, a plug-and-play flow module is developed to introduce on-demand microfluidic flow capabilities to an open-well device that features a nanoporous membrane and live-cell imaging capabilities. The magnetic latching assembly of this design enables bi-directional reconfiguration and allows users to conduct an experiment in an open-well format with established protocols and then add or remove microfluidic capabilities as desired. This work also provides an experimentally-validated flow model to select flow conditions based on the experimental needs. As a proof-of-concept, flow-induced alignment of endothelial cells and the expression of shear-sensitive gene targets are demonstrated, and the different phases of neutrophil transmigration across a chemically stimulated endothelial monolayer under flow conditions are visualized. With these experimental capabilities, it is anticipated that both engineering and bioscience laboratories will adopt this reconfigurable design due to the compatibility with standard open-well protocols.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.