ArticleThe Journal of clinical investigation2022
In vivo visualization and molecular targeting of the cardiac conduction system.
Article in The Journal of clinical investigation, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 29 citations in OpenAlex.
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- Prioritizing Discovery and Advancements in Arrhythmia Therapies: NIH/NHLBI Workshop.JACC. Clinical electrophysiology · 2026Review
- Spatial transcriptomic profiling of developing mouse hearts reveals a spatially patterned signaling environment.Communications biology · 2026Article
- The cardiac conduction system: development, function and therapeutic targets.Nature reviews. Cardiology · 2026Review
- New Perspectives Provided by Merging Computed Tomographic Scanning and Electroanatomical Mapping of Koch's Pyramid.Journal of cardiovascular development and disease · 2026Article
- Microimager: a flexible thin-film miniaturized endoscope for optical biomedical imaging.Biomedical optics express · 2025Article
- Aging-induced changes in lymphatic muscle cell transcriptomes are associated with reduced pumping of peripheral collecting lymphatic vessels in mice.Developmental cell · 2025Article
- Unveiling the native architecture of adult cardiac tissue using the 3D-NaissI method.Cellular and molecular life sciences : CMLS · 2025Article
- Development of an optimized cell-based selection system for phage display libraries.Biology methods & protocols · 2025Article
- Top stories on advances in understanding ventricular conduction system development, physiology, arrhythmogenesis, and therapeutics.Heart rhythm · 2024Article
- Single-cell transcriptome analysis reveals CD34 as a marker of human sinoatrial node pacemaker cardiomyocytes.Nature communications · 2024Article
- Transcriptional regulation of the postnatal cardiac conduction system heterogeneity.Nature communications · 2024Article
- Cardiac conduction diseases: understanding the molecular mechanisms to uncover targets for future treatments.Expert opinion on therapeutic targets · 2024Review
- Review
- DbhNature communications · 2023Article
- Review
- Unwind to the beat: chromatin and cardiac conduction.The Journal of clinical investigation · 2023Article
- Revolutionizing cardiovascular medicine: targeted therapies for the cardiac conduction system.The Journal of clinical investigation · 2022Article
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Authors and funding
16 authors at 6 institutions in 4 countries.
Funding
Abstract
Accidental injury to the cardiac conduction system (CCS), a network of specialized cells embedded within the heart and indistinguishable from the surrounding heart muscle tissue, is a major complication in cardiac surgeries. Here, we addressed this unmet need by engineering targeted antibody-dye conjugates directed against the CCS, allowing for the visualization of the CCS in vivo following a single intravenous injection in mice. These optical imaging tools showed high sensitivity, specificity, and resolution, with no adverse effects on CCS function. Further, with the goal of creating a viable prototype for human use, we generated a fully human monoclonal Fab that similarly targets the CCS with high specificity. We demonstrate that, when conjugated to an alternative cargo, this Fab can also be used to modulate CCS biology in vivo, providing a proof of principle for targeted cardiac therapeutics. Finally, in performing differential gene expression analyses of the entire murine CCS at single-cell resolution, we uncovered and validated a suite of additional cell surface markers that can be used to molecularly target the distinct subcomponents of the CCS, each prone to distinct life-threatening arrhythmias. These findings lay the foundation for translational approaches targeting the CCS for visualization and therapy in cardiothoracic surgery, cardiac imaging, and arrhythmia management.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.