Evidence map›Paper›PMID 35951301›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Determination of Effects and Mechanisms of Action of Bacterial Amyloids on Antibiotic Resistance.

Krzysztof Kubiak, Lidia Gaffke, Karolina Pierzynowska, Zuzanna Cyske, Łukasz Grabowski, Katarzyna Kosznik-Kwaśnicka, Weronika Jaroszewicz, Alicja Węgrzyn, Grzegorz Węgrzyn

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
3.3field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Krzysztof Kubiak *Department of Molecular Biology, University of Gdansk, Gdansk, Poland.
Lidia Gaffke *Department of Molecular Biology, University of Gdansk, Gdansk, Poland.
Karolina Pierzynowska *Department of Molecular Biology, University of Gdansk, Gdansk, Poland.
Zuzanna Cyske *Department of Molecular Biology, University of Gdansk, Gdansk, Poland.
Łukasz GrabowskiLaboratory of Phage Therapy, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Gdansk, Poland.
Katarzyna Kosznik-KwaśnickaLaboratory of Phage Therapy, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Gdansk, Poland.
Weronika JaroszewiczDepartment of Molecular Biology, University of Gdansk, Gdansk, Poland.
Alicja WęgrzynLaboratory of Phage Therapy, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Gdansk, Poland.
Grzegorz WęgrzynDepartment of Molecular Biology, University of Gdansk, Gdansk, Poland. grzegorz.wegrzyn@ug.edu.pl.
University of Gdańsk · PLInstitute of Biochemistry and Biophysics, Polish Academy of Sciences · PL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bacterial functional amyloids, apart from their many other functions, can influence the resistance of bacteria to antibiotics and other antibacterial agents. Mechanisms of modulation of susceptibility of bacterial cells to antimicrobials can be either indirect or direct. The former mechanisms are exemplified by the contribution of functional amyloids to biofilm formation, which may effectively prevent the penetration of various compounds into bacterial cells. The direct mechanisms include the effects of bacterial proteins revealing amyloid-like structures, like the C-terminal region of the Escherichia coli Hfq protein, on the expression of genes involved in antibiotic resistance. Therefore, in this paper, we describe methods by which effects and mechanisms of action of bacterial amyloids on antibiotic resistance can be studied. Assessment of formation of biofilms, determination of the efficiency of antibiotic resistance in solid and liquid media, and determination of the effects on gene expression at levels of mRNA abundance and stability and protein abundance are described.

Indexed as

BiofilmsEscherichia coliAmyloidAnti-Bacterial AgentsBacteriaBacterial ProteinsDrug Resistance, BacterialDrug Resistance, MicrobialAmyloidAnti-Bacterial AgentsBacterial ProteinsAmyloids in antibiotic resistanceBiofilmMinimal inhibitory concentration (MIC)mRNA abundance and stabilityProtein level determinationWES—automatic Western blotting system

Identifiers

PMID35951301
OpenAlexW4291021662

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.