Evidence map›Paper›PMID 35951298›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

High-Throughput Screening of Heterologous Functional Amyloids Using Escherichia coli.

Elizabeth A Yates, Luis A Estrella, Christopher R So

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 67% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Elizabeth A YatesDepartment of Chemistry, US Naval Academy, Annapolis, MD, USA.
Luis A EstrellaFormerly Chemistry Division, US Naval Research Laboratory, Washington, DC, USA.
Christopher R SoChemistry Division, US Naval Research Laboratory, Washington, DC, USA. christopher.so@nrl.navy.mil.
United States Naval Research Laboratory · USUnited States Naval Academy · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Escherichia coli remains one of the most widely used workhorse microorganisms for the expression of heterologous proteins. The large number of cloning vectors and mutant host strains available for E. coli yields an impressively wide array of folded globular proteins in the laboratory. However, applying modern functional screening approaches to interrogate insoluble protein aggregates such as amyloids requires the use of nonstandard expression pathways. In this chapter, we detail the use of the curli export pathway in E. coli to express a library of gene fragments and variants of a functional amyloid protein to screen sequence traits responsible for aggregation and the formation of nanoscale materials.

Indexed as

Escherichia coli InfectionsEscherichia coli ProteinsAmyloidBacterial ProteinsBiofilmsEscherichia coliHigh-Throughput Screening AssaysHumansAmyloidBacterial ProteinsEscherichia coli ProteinsBacterial screeningBiofilmFunctional amyloidProtein engineeringSelf-assembly

Identifiers

PMID35951298
OpenAlexW4290973486

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.