ArticleMicrobiology spectrum2022
Comparative Assessment of Filtration- and Precipitation-Based Methods for the Concentration of SARS-CoV-2 and Other Viruses from Wastewater.
Article in Microbiology spectrum, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 68 citations in OpenAlex.
- Pooled it
- Surface-Enhanced Raman Spectroscopy for Viral Diagnostics: Principles, Strategies, Clinical Challenges, and Future Directions.Chemical reviews · 2026Review
- Article
- Comparative virome analysis of lake and domestic wastewater revealed the unexpected presence of swine acute diarrhea syndrome coronavirus andFrontiers in microbiology · 2026Article
- Benchmarking Concentration and Extraction Methods for Wastewater-Based Surveillance of Eight Human Respiratory Viruses: Implications for Rapid Application to Novel Pathogens.Environmental science & technology · 2025Article
- Towards ultra-sensitive and rapid near-source wastewater-based epidemiology.Nature communications · 2025Article
- Trends and Challenges in the Detection and Environmental Surveillance of the Hepatitis E Virus.Microorganisms · 2025Review
- Phage therapeutic delivery methods and clinical trials for combating clinically relevant pathogens.Therapeutic delivery · 2025Review
- The Impact of Viral Concentration Method on Quantification and Long Amplicon Nanopore Sequencing of SARS-CoV-2 and Noroviruses in Wastewater.Microorganisms · 2025Article
- Evaluation of Three Viral Capsid Integrity qPCR Methods for Wastewater-Based Viral Surveillance.Food and environmental virology · 2025Article
- Monitoring of antimicrobial resistance genes and influenza viruses in avian-populated water bodies.Sustainable microbiology · 2025Article
- Magnetic Carbon Bead-Based Concentration Method for SARS-CoV-2 Detection in Wastewater.Food and environmental virology · 2024Article
- Assessment of Skimmed Milk Flocculation for Bacterial Enrichment from Water Samples, and Benchmarking of DNA Extraction and 16S rRNA Databases for Metagenomics.International journal of molecular sciences · 2024Article
- Tracking epidemic viruses in wastewaters.Microbial biotechnology · 2024Review
- Evaluation of an Automated Ultrafiltration System for Concentrating a Range of Viruses from Saline Waters.Food and environmental virology · 2024Article
- Article
- Simple SARS-CoV-2 concentration methods for wastewater surveillance in low resource settings.The Science of the total environment · 2024Article
- Near-source passive sampling for monitoring viral outbreaks within a university residential setting.Epidemiology and infection · 2024Article
- Comparative assessment of Nanotrap and polyethylene glycol-based virus concentration in wastewater samples.FEMS microbes · 2024Article
- Article
Corrections and comments
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Authors and funding
13 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Wastewater-based epidemiology (WBE) has been widely used to track levels of SARS-CoV-2 infection in the community during the COVID-19 pandemic. Due to the rapid expansion of WBE, many methods have been used and developed for virus concentration and detection in wastewater. However, very little information is available on the relative performance of these approaches. In this study, we compared the performance of five commonly used wastewater concentration methods for the detection and quantification of pathogenic viruses (SARS-CoV-2, norovirus, rotavirus, influenza, and measles viruses), fecal indicator viruses (crAssphage, adenovirus, pepper mild mottle virus), and process control viruses (murine norovirus and bacteriophage Phi6) in laboratory spiking experiments. The methods evaluated included those based on either ultrafiltration (Amicon centrifugation units and InnovaPrep device) or precipitation (using polyethylene glycol [PEG], beef extract-enhanced PEG, and ammonium sulfate). The two best methods were further tested on 115 unspiked wastewater samples. We found that the volume and composition of the wastewater and the characteristics of the target viruses greatly affected virus recovery, regardless of the method used for concentration. All tested methods are suitable for routine virus concentration; however, the Amicon ultrafiltration method and the beef extract-enhanced PEG precipitation methods yielded the best recoveries. We recommend the use of ultrafiltration-based concentration for low sample volumes with high virus titers and ammonium levels and the use of precipitation-based concentration for rare pathogen detection in high-volume samples.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.