Evidence map›Paper›PMID 35950846›Full record

ArticleMicrobiology spectrum2022

High-Throughput COVID-19 Testing of Naso-Oropharyngeal Swabs Using a Sensitive Extraction-Free Sample Preparation Method.

Todd M Pryce, Erin J Haygarth, Jessica Bordessa, Courtney T Jeffery, Ian D Kay, James P Flexman, Peter A Boan

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiology spectrum, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 49% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Todd M PrycePathWest Laboratory Medicine WA, Department of Clinical Microbiology, Fiona Stanley Hospital, Murdoch, Western Australia, Australia.ORCID 0000-0002-5293-9795
Erin J HaygarthPathWest Laboratory Medicine WA, Department of Clinical Microbiology, Fiona Stanley Hospital, Murdoch, Western Australia, Australia.
Jessica BordessaPathWest Laboratory Medicine WA, Department of Clinical Microbiology, Fiona Stanley Hospital, Murdoch, Western Australia, Australia.
Courtney T JefferyPathWest Laboratory Medicine WA, Department of Clinical Microbiology, Fiona Stanley Hospital, Murdoch, Western Australia, Australia.
Ian D KayPathWest Laboratory Medicine WA, Department of Clinical Microbiology, Fiona Stanley Hospital, Murdoch, Western Australia, Australia.
James P FlexmanPathWest Laboratory Medicine WA, Department of Clinical Microbiology, Fiona Stanley Hospital, Murdoch, Western Australia, Australia.
Peter A BoanPathWest Laboratory Medicine WA, Department of Clinical Microbiology, Fiona Stanley Hospital, Murdoch, Western Australia, Australia.ORCID 0000-0002-0518-0421
Fiona Stanley Hospital · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

High-throughput diagnostic assays are required for large-scale population testing for severe acute respiratory coronavirus 2 (SARS-CoV-2). The gold standard technique for SARS-CoV-2 detection in nasopharyngeal swab specimens is nucleic acid extraction followed by real-time reverse transcription-PCR. Two high-throughput commercial extraction and detection systems are used routinely in our laboratory: the Roche cobas SARS-CoV-2 assay (cobas) and the Roche MagNA Pure 96 system combined with the SpeeDx PlexPCR SARS-CoV-2 assay (Plex). As an alternative to more costly instrumentation, or tedious sample pooling to increase throughput, we developed a high-throughput extraction-free sample preparation method for naso-oropharyngeal swabs using the PlexPCR SARS-CoV-2 assay (Direct). A collection of SARS-CoV-2-positive (

Indexed as

COVID-19COVID-19 TestingHumansNasopharynxRNA, ViralSARS-CoV-2Specimen HandlingRNA, Viralcobasextraction-freehigh-throughputPlexPCRSARS-CoV-2

Identifiers

PMID35950846
PMCPMC9430511
OpenAlexW4290804108

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.