Evidence map›Paper›PMID 35949424›Full record

ArticleSynthetic biology (Oxford, England)2022

Multicolor plate reader fluorescence calibration.

Jacob Beal, Cheryl A Telmer, Alejandro Vignoni, Yadira Boada, Geoff S Baldwin, Liam Hallett, Taeyang Lee, Vinoo Selvarajah, Sonja Billerbeck, Bradley Brown and 11 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Synthetic biology (Oxford, England), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
2.6field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 10 citations in OpenAlex.

  1. Construction of aACS omega · 2025
    Article
  2. Article
  3. Article
  4. Special issue: reproducibility in synthetic biology.Synthetic biology (Oxford, England) · 2023
    Article
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors at 12 institutions in 6 countries.

Jacob BealIntelligent Software and Systems, Raytheon BBN Technologies, 10 Moulton Street, Cambridge 02138, MA, USA.ORCID https://orcid.org/0000-0002-1663-5102
Cheryl A TelmerDepartment of Biological Sciences, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh 15213, PA, USA.ORCID https://orcid.org/0000-0002-7649-3134
Alejandro VignoniSynthetic Biology and Biosystems Control Group, Instituto de Automatica e Informatica Industrial, Universitat Politecnica de Valencia, Camino de Vera s/n, Valencia 46022, Spain.ORCID https://orcid.org/0000-0001-9977-7132
Yadira BoadaSynthetic Biology and Biosystems Control Group, Instituto de Automatica e Informatica Industrial, Universitat Politecnica de Valencia, Camino de Vera s/n, Valencia 46022, Spain.
Geoff S BaldwinDepartment of Life Sciences, Imperial College London, South Kensington Campus, Exhibition Road, London SW7 2AZ, UK.ORCID https://orcid.org/0000-0003-4800-023X
Liam HallettDepartment of Life Sciences, Imperial College London, South Kensington Campus, Exhibition Road, London SW7 2AZ, UK.
Taeyang LeeDepartment of Life Sciences, Imperial College London, South Kensington Campus, Exhibition Road, London SW7 2AZ, UK.
Vinoo SelvarajahiGEM Foundation, 45 Prospect Street, Cambridge 02139, MA, USA.
Sonja BillerbeckMolecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 7, Groningen 9747 AG, The Netherlands.ORCID https://orcid.org/0000-0002-3092-578X
Bradley BrownSchool of Engineering, Newcastle University, Devonshire Building, Devonshire Terrace, NE1 7RU Newcastle Upon Tyne, UK.
Guo-Nan CaiSchool of Life Sciences, Fudan University, 220 Handan Road, Shanghai 200433, China.
Liang CaiSchool of Life Sciences, Fudan University, 220 Handan Road, Shanghai 200433, China.
Edward EisensteinInstitute of Bioscience and Biotechnology Research, Fischell Department of Bioengineering, University of Maryland, 9600 Gudelsky Drive, Rockville 20850, MD, USA.
Daisuke KigaSchool of Advanced Science and Engineering, Waseda University, 2-2 Wakamatsu Cho, Totsukamachi, Shinjuku City 169-8050, Tokyo, Japan.
David RossMaterial Measurement Laboratory, National Institute of Standards and Technology, 100 Bureau Dr., Gaithersburg 20899, MD, USA.ORCID https://orcid.org/0000-0002-7790-218X
Nina AlperovichMaterial Measurement Laboratory, National Institute of Standards and Technology, 100 Bureau Dr., Gaithersburg 20899, MD, USA.
Noah SprentDepartment of Chemical Engineering, Imperial College London, South Kensington Campus, Exhibition Road, London SW7 2AZ, UK.ORCID https://orcid.org/0000-0001-9129-9236
Jaclyn ThompsonChemical Engineering, Worcester Polytechnic Institute, 100 Institute Road, Worcester 01609-2280, MA, USA.
Eric M YoungChemical Engineering, Worcester Polytechnic Institute, 100 Institute Road, Worcester 01609-2280, MA, USA.
Drew EndyBioengineering, Stanford University, 443 Via Ortega, Stanford 94305, CA, USA.
Traci Haddock-AngelliiGEM Foundation, 45 Prospect Street, Cambridge 02139, MA, USA.
Imperial College London · GBFudan University · CNNational Institute of Standards and Technology · USUniversitat Politècnica de València · ESWorcester Polytechnic Institute · USAdvanced Bioscience Laboratories (United States) · USCarnegie Mellon University · USNewcastle University · GBRTX (United States) · USStanford University · USUniversity of Groningen · NLWaseda University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Plate readers are commonly used to measure cell growth and fluorescence, yet the utility and reproducibility of plate reader data is limited by the fact that it is typically reported in arbitrary or relative units. We have previously established a robust serial dilution protocol for calibration of plate reader measurements of absorbance to estimated bacterial cell count and for green fluorescence from proteins expressed in bacterial cells to molecules of equivalent fluorescein. We now extend these protocols to calibration of red fluorescence to the sulforhodamine-101 fluorescent dye and blue fluorescence to Cascade Blue. Evaluating calibration efficacy via an interlaboratory study, we find that these calibrants do indeed provide comparable precision to the prior calibrants and that they enable effective cross-laboratory comparison of measurements of red and blue fluorescence from proteins expressed in bacterial cells.

Indexed as

calibrationcell countfluorescenceunits

Identifiers

PMID35949424
PMCPMC9357555
OpenAlexW4288038488

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.