ArticleJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc2022
Considerations in the use of processing fluids for the detection of PRRSV RNA and antibody.
Article in Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Testicular processing fluid as a useful matrix for the detection of porcine circovirus type 2 DNA and virus-specific antibodies.Frontiers in veterinary science · 2026Article
- Characterizing dynamics of PRRSV RNA positivity over time in sows after live virus exposure and associated risk factors based on TOSc samples.BMC veterinary research · 2025Article
- Field study on the utility of fluid obtained from testicles as a sample for detecting antibodies to selected swine pathogens.Scientific reports · 2025Article
- PRRSV RNA Detection and Predictive Values Between Different Sow and Neonatal Litter Sample Types.Veterinary sciences · 2025Article
- Effect of Time and Temperature on the Detection of PRRSV RNA and Endogenous Internal Sample Control in Porcine Tongue Fluids.Veterinary sciences · 2025Article
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Authors and funding
12 authors.
Funding
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Abstract
Surveillance is mandatory for tracking the progress of porcine reproductive and respiratory syndrome virus (PRRSV) control and elimination efforts in breeding herds. Processing fluids, the fluid recovered from tissues collected at castration and/or tail docking, are used for breeding herd surveillance by large segments of the industry, but the basic diagnostic characteristics of processing fluids are largely undescribed. We undertook 3 studies to address this information gap. In study 1, we found no differences among the PRRSV RT-rtPCR results obtained with 4 commercial RNA extraction kits. In study 2, we found that PRRSV RNA was highly stable in processing fluid samples at -20°C or 4°C, but detrimental effects were observed at ≥22°C within 24 h. In study 3, using a modified PRRSV ELISA at a sample:positive cutoff of ≥0.5, we found excellent discrimination in the detection of PRRSV antibody (IgM, IgA, IgG) in processing fluids from herds of known PRRSV status. Judicious handling of processing fluid samples from sow herds, and the use of methods available in veterinary diagnostic laboratories, can provide a foundation for reliable PRRSV surveillance.
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