Evidence map›Paper›PMID 35948517›Full record

ArticleChemistry (Weinheim an der Bergstrasse, Germany)2022

YfeX - A New Platform for Carbene Transferase Development with High Intrinsic Reactivity.

Victor Sosa Alfaro, Sodiq O Waheed, Hannah Palomino, Anja Knorrscheidt, Martin Weissenborn, Christo Z Christov, Nicolai Lehnert

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chemistry (Weinheim an der Bergstrasse, Germany), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Victor Sosa AlfaroDepartment of Chemistry and Department of Biophysics, University of Michigan, Ann Arbor, Michigan, 48109-1055, United States.ORCID http://orcid.org/0000-0002-4481-0246
Sodiq O WaheedDepartment of Chemistry, Michigan Technological University, Houghton, Michigan, 49931, United States.ORCID http://orcid.org/0000-0003-3422-4531
Hannah PalominoDepartment of Chemistry and Department of Biophysics, University of Michigan, Ann Arbor, Michigan, 48109-1055, United States.
Anja KnorrscheidtInstitute of Chemistry, Martin-Luther-University Halle-Wittenberg, Kurt-Mothes-Str. 2, 06120, Halle, Germany.
Martin WeissenbornInstitute of Chemistry, Martin-Luther-University Halle-Wittenberg, Kurt-Mothes-Str. 2, 06120, Halle, Germany.
Christo Z ChristovDepartment of Chemistry, Michigan Technological University, Houghton, Michigan, 49931, United States.
Nicolai LehnertDepartment of Chemistry and Department of Biophysics, University of Michigan, Ann Arbor, Michigan, 48109-1055, United States.ORCID http://orcid.org/0000-0002-5221-5498

Funding

Understanding the Ligand Binding by Non-Heme Fe(II)- and 2-Oxoglutarate-Dependent Histone DemethylasesR15GM139118 · NIGMS · MICHIGAN TECHNOLOGICAL UNIVERSITY · PI CHRISTOV, CHRISTO ZHIVKOV · 2021 to 2024
$786k
NIGMS NIH HHS R15 GM139118US-NIH 1R15GM139118-01A1US-NSF 1904215
6 · The paper itself

Abstract

Carbene transfer biocatalysis has evolved from basic science to an area with vast potential for the development of new industrial processes. In this study, we show that YfeX, naturally a peroxidase, has great potential for the development of new carbene transferases, due to its high intrinsic reactivity, especially for the N-H insertion reaction of aromatic and aliphatic primary and secondary amines. YfeX shows high stability against organic solvents (methanol and DMSO), greatly improving turnover of hydrophobic substrates. Interestingly, in styrene cyclopropanation, WT YfeX naturally shows high enantioselectivity, generating the trans product with 87 % selectivity for the (R,R) enantiomer. WT YfeX also catalyzes the Si-H insertion efficiently. Steric effects in the active site were further explored using the R232A variant. Quantum Mechanics/Molecular Mechanics (QM/MM) calculations reveal details on the mechanism of Si-H insertion. YfeX, and potentially other peroxidases, are exciting new targets for the development of improved carbene transferases.

Indexed as

MethaneTransferasesBiocatalysisCatalytic DomainPeroxidasescarbeneMethanePeroxidasesTransferasesbiocatalysiscarbene transfercyclopropanationN−H insertionQM/MM calculations

Identifiers

PMID35948517
PMCPMC9691539

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.