ArticleACS synthetic biology2022
Actinosomes: Condensate-Templated Containers for Engineering Synthetic Cells.
Article in ACS synthetic biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Construction of artificial organellesChemical science · 2025Review
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- Phase separation and ageing of glycine-rich protein from tick adhesive.Nature chemistry · 2025Article
- Elastin-like polypeptide coacervates as reversibly triggerable compartments for synthetic cells.Communications chemistry · 2024Article
- 2D and 3D Self-Assembly of Fluorine-Free Pillar-[5]-Arenes and Perfluorinated Diacids at All-Aqueous Interfaces.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Programmable Enzymatic Reaction Network in Artificial Cell-Like Polymersomes.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Building Synthetic Cells─From the Technology Infrastructure to Cellular Entities.ACS synthetic biology · 2024Review
- Artificial cell synthesis using biocatalytic polymerization-induced self-assembly.Nature chemistry · 2024Article
- Synthetic Cells Revisited: Artificial Cells Construction Using Polymeric Building Blocks.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- pH-Responsive Elastin-Like Polypeptide Designer Condensates.ACS applied materials & interfaces · 2023Article
- Biomimetic behaviors in hydrogel artificial cells through embedded organelles.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- In Vitro Transcription-Translation in an Artificial Biomolecular Condensate.ACS synthetic biology · 2023Article
- What remains from living cells in bacterial lysate-based cell-free systems.Computational and structural biotechnology journal · 2023Review
- Methods to mechanically perturb and characterize GUV-based minimal cell models.Computational and structural biotechnology journal · 2023Review
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Authors and funding
3 authors.
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Abstract
Engineering synthetic cells has a broad appeal, from understanding living cells to designing novel biomaterials for therapeutics, biosensing, and hybrid interfaces. A key prerequisite to creating synthetic cells is a three-dimensional container capable of orchestrating biochemical reactions. In this study, we present an easy and effective technique to make cell-sized porous containers, coined actinosomes, using the interactions between biomolecular condensates and the actin cytoskeleton. This approach uses polypeptide/nucleoside triphosphate condensates and localizes actin monomers on their surface. By triggering actin polymerization and using osmotic gradients, the condensates are transformed into containers, with the boundary made up of actin filaments and polylysine polymers. We show that the guanosine triphosphate (GTP)-to-adenosine triphosphate (ATP) ratio is a crucial parameter for forming actinosomes: insufficient ATP prevents condensate dissolution, while excess ATP leads to undesired crumpling. Permeability studies reveal the porous surface of actinosomes, allowing small molecules to pass through while restricting bigger macromolecules within the interior. We show the functionality of actinosomes as bioreactors by carrying out
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