Evidence map›Paper›PMID 35947212›Full record

ArticleProtoplasma2023

Anther development in Brachiaria brizantha (syn. Urochloa brizantha) and perspective for microspore in vitro culture.

Andréa D Koehler, Mônica L Rossi, Vera T C Carneiro, Glaucia B Cabral, Adriana P Martinelli, Diva M A Dusi

Abstract read
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In one paragraph

Article in Protoplasma, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.4field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Andréa D KoehlerUniversity of Sao Paulo, CENA, Av. Centenario 303, Piracicaba, SP, 13416-903, Brazil.
Mônica L RossiUniversity of Sao Paulo, CENA, Av. Centenario 303, Piracicaba, SP, 13416-903, Brazil.
Vera T C CarneiroBrazilian Agricultural Research Corporation (Embrapa), Embrapa Genetic Resources and Biotechnology, Cx.Postal 02372, Brasilia, DF, 70.770-917, Brazil.
Glaucia B CabralBrazilian Agricultural Research Corporation (Embrapa), Embrapa Genetic Resources and Biotechnology, Cx.Postal 02372, Brasilia, DF, 70.770-917, Brazil.
Adriana P MartinelliUniversity of Sao Paulo, CENA, Av. Centenario 303, Piracicaba, SP, 13416-903, Brazil.
Diva M A DusiBrazilian Agricultural Research Corporation (Embrapa), Embrapa Genetic Resources and Biotechnology, Cx.Postal 02372, Brasilia, DF, 70.770-917, Brazil. diva.dusi@embrapa.br.ORCID http://orcid.org/0000-0001-9480-0460
Brazilian Agricultural Research Corporation · BRUniversidade de São Paulo · BR

Funding

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior 001Coordenação de Aperfeiçoamento de Pessoal de Nível Superior 141959/2006-1Coordenação de Aperfeiçoamento de Pessoal de Nível Superior 305785/2008-7
6 · The paper itself

Abstract

Brachiaria, a genus from the Poaceae family, is largely cultivated as forage in Brazil. Among the most cultivated varieties of Brachiaria spp., B. brizantha cv. Marandu (syn. Urochloa brizantha) is of great agronomical importance due to the large areas cultivated with this species. This cultivar is apomictic and tetraploid. Sexual diploid genotype is available for this species. The difference in levels of ploidy among sexual and apomictic plants contributes to hindering Brachiaria breeding programs. The induction of haploids and double haploids is of great interest for the generation of new genotypes with potential use in intraspecific crosses. A key factor for the success of this technique is identifying adequate microspore developmental stages for efficient embryogenesis induction. Knowledge of the morphological changes during microsporogenesis and microgametogenesis and sporophytic tissues composing the anther is critical for identifying the stages in which microspores present a higher potential for embryogenic callus and somatic embryo through in vitro culture. In this work, morphological markers were associated with anther and pollen grain developmental stages, through histological analysis. Anther development was divided into 11 stages using morphological and cytological characteristics, from anther with archesporial cells to anther dehiscence. The morphological characteristics of each stage are presented. In addition, the response of stage 8 anthers to in vitro culture indicates microspores initiating somatic embryogenic pathway.

Indexed as

BrachiariaPlant BreedingPoaceaeReproductionTetraploidyIn vitro microspore embryogenesisMale gametophyteMicrogametogenesisMicrosporogenesisPoaceaeUltrastructure

Identifiers

PMID35947212
OpenAlexW4292470322

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.