Evidence map›Paper›PMID 35946163›Full record

ArticleJournal of the Royal Society, Interface2022

RecA and RecB: probing complexes of DNA repair proteins with mitomycin C in live

Alex L Payne-Dwyer, Aisha H Syeda, Jack W Shepherd, Lewis Frame, Mark C Leake

Open access · greenAbstract read
In one paragraph

Article in Journal of the Royal Society, Interface, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
2.2field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 25 citations in OpenAlex.

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  14. Response ofMicroorganisms · 2024
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Alex L Payne-DwyerDepartment of Physics, University of York, York YO10 5DD, UK.ORCID 0000-0001-8802-352X
Aisha H SyedaDepartment of Physics, University of York, York YO10 5DD, UK.
Jack W ShepherdDepartment of Physics, University of York, York YO10 5DD, UK.
Lewis FrameSchool of Natural Sciences, University of York, York YO10 5DD, UK.
Mark C LeakeDepartment of Physics, University of York, York YO10 5DD, UK.ORCID 0000-0002-1715-1249
University of York · GB

Funding

Biotechnology and Biological Sciences Research Council BB/N006453/1Biotechnology and Biological Sciences Research Council BB/P000746/1
6 · The paper itself

Abstract

The RecA protein and RecBCD complex are key bacterial components for the maintenance and repair of DNA. RecBCD is a helicase-nuclease that uses homologous recombination to resolve double-stranded DNA breaks. It also facilitates coating of single-stranded DNA with RecA to form RecA filaments, a vital step in the double-stranded break DNA repair pathway. However, questions remain about the mechanistic roles of RecA and RecBCD in live cells. Here, we use millisecond super-resolved fluorescence microscopy to pinpoint the spatial localization of fluorescent reporters of RecA or RecB at physiological levels of expression in individual live

Indexed as

Escherichia coliEscherichia coli ProteinsDNADNA, BacterialDNA RepairDNA, Single-StrandedExodeoxyribonuclease VMitomycinRecombination, GeneticDNADNA, BacterialDNA, Single-StrandedEscherichia coli ProteinsExodeoxyribonuclease VMitomycinDNA damagemitomycin Crecombinationrepairsingle-molecule trackingsuper-resolution microscopy

Identifiers

PMID35946163
PMCPMC9363994
OpenAlexW3204253085

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.