ArticleJournal of applied microbiology2022
A novel, magnetic bead-based extraction method for the isolation of antimicrobial resistance genes with a case study in river water in Malawi.
Article in Journal of applied microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed.
- Near-real-time whole-genome sequencing in hospital infection control: a narrative review.Infection · 2026Review
- Development and pilot application of a point-of-need molecular xenomonitoring protocol for tsetse (Glossina sp.) in a low-resource setting.PLoS neglected tropical diseases · 2026Article
- Comparison of boiling versus magnetic bead techniques in nucleic acid extraction for human papillomavirus detection: evidence based 17,179 cases.Virology journal · 2025Article
- Magnetic Nanoparticle-Based DNA Isolation from Refined Soybean Oil: A Cost-Effective Approach for GM Testing.Foods (Basel, Switzerland) · 2025Article
- Complete genome sequence ofMicrobiology resource announcements · 2024Article
- Stray Magnetic Field Variations and Micromagnetic Simulations: Models for NiMicromachines · 2024Article
- Carbapenem Resistance in Animal-Environment-Food from Africa: A Systematic Review, Recommendations and Perspectives.Infection and drug resistance · 2024Review
- Antimicrobial resistance detection methods in water environments: a scoping review.Sustainable microbiology · 2024Review
- Research on a Magnetic Separation-Based Rapid Nucleic Acid Extraction System and Its Detection Applications.Biosensors · 2023Article
- A novel, magnetic bead-based extraction method for the isolation of antimicrobial resistance genes with a case study in river water in Malawi.Journal of applied microbiology · 2022Article
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Authors and funding
12 authors.
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Abstract
aimsThe environment is increasingly recognized as an important reservoir of antimicrobial resistance genes (ARGs), which can be identified using molecular platforms. Yet, environmental surveillance remains an underutilised tool as there is no agreement on the best strategy for sample processing. We aim to develop a low-cost extraction method independent to commercial kits or reagents. METHODS AND
resultsWe present a novel, magnetic bead-based method for the isolation of ARGs from river water named MagnaExtract. We present this with analytic limit of detection as well as a case study in Southern Malawi. Here we compare the DNA yield from MagnaExtract with commercially available QIAGEN kits and the crude boil and spin method, using a high-resolution melt analysis PCR panel designed for the detection of third-generation cephalosporin and carbapenem-resistant genes from 98 water samples.
conclusionThe MagnaExtract method is comparable, and in some instance's superior to commercially available kits for the isolation of ARGs from river water samples. SIGNIFICANCE AND IMPACT OF THE STUDY: The MagnaExtract approach offers a simple, affordable, high yielding extraction method that could be used for the detection of ARGs from river water samples in surveillance campaigns in East Africa.
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