Evidence map›Paper›PMID 35945269›Full record

ArticleLaboratory investigation; a journal of technical methods and pathology2022

CircRNA-ST6GALNAC6 increases the sensitivity of bladder cancer cells to erastin-induced ferroptosis by regulating the HSPB1/P38 axis.

Li Wang, Shuiqing Wu, Haiqing He, Kai Ai, Ran Xu, Lei Zhang, Xuan Zhu

Open access · bronzeAbstract read
PubMed Publisher
In one paragraph

Article in Laboratory investigation; a journal of technical methods and pathology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.

0numbers the graph read from it
0cells of the map it votes in
47citing papers in PubMed
5.8field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

47 citing papers in PubMed, 66 citations in OpenAlex.

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  13. CircDUSP22 Attenuates the Ferroptosis of Prostate Cancer CellsCombinatorial chemistry & high throughput screening · 2025
    Article
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  20. Molecular mechanisms of ferroptosis and its effects on bladder cancer.Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences · 2024
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Li WangDepartment of Thoracic Surgery, The Second Xiangya Hospital of Central South University, Changsha, 410013, Hunan, PR China.
Shuiqing WuDepartment of Urology, The Second Xiangya Hospital of Central South University, Changsha, 410013, Hunan, PR China.
Haiqing HeDepartment of Urology, The Second Xiangya Hospital of Central South University, Changsha, 410013, Hunan, PR China.
Kai AiDepartment of Urology, The Second Xiangya Hospital of Central South University, Changsha, 410013, Hunan, PR China.
Ran XuDepartment of Urology, The Second Xiangya Hospital of Central South University, Changsha, 410013, Hunan, PR China.
Lei ZhangDepartment of Urology, The Second Xiangya Hospital of Central South University, Changsha, 410013, Hunan, PR China.
Xuan ZhuDepartment of Urology, The Second Xiangya Hospital of Central South University, Changsha, 410013, Hunan, PR China. zhuxuan@csu.edu.cn.ORCID 0000-0002-4404-4393
Central South University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Previous studies have demonstrated that circST6GALNAC6 is a tumor suppressor in bladder cancer. However, the role of circST6GALNAC6 in ferroptosis remains unclear. In the current study, ferroptosis was induced in bladder cancer cells by erastin. Functional experiments showed that overexpression of circST6GALNAC6 promoted ferroptosis of bladder cancer cells in vitro and in vivo. Mechanistic studies revealed that circST6GALNAC6 bound to the N-terminus of small heat shock protein 1 (HSPB1) and thus blocked the erastin-induced phosphorylation of HSPB1 at the Ser-15 site, a phosphorylation site in the protective response to ferroptosis stress. In addition, protein kinase C inhibited circST6GALNAC6-induced ferroptosis by increasing the overall phosphorylation level of HSPB1, further demonstrating the role of phosphorylation activation of HSPB1 in resistance to ferroptosis. Finally, the involvement of the HSPB1/p38 MAPK pathway in the downstream signal transduction of circST6GALNAC6 in bladder cancer ferroptosis regulation was determined. The regulatory mechanism of ferroptosis sensitivity dependent on circST6GALNAC6 expression levels in bladder cancer was revealed as circRNA regulation of various protein functions. CircST6GALNAC6 inhibits HSPB1 and promotes cell ferroptosis by occupying the phosphorylation site (Ser-15) of HSBP1 and activating the P38 MAPK signaling pathway. Therefore, enhancing the expression of circST6GALNAC6 to promote ferroptosis or using circST6GALNAC6 as a biomarker of ferroptosis sensitivity is of considerable importance to the development and application of ferroptosis intervention methods in bladder cancer.

Indexed as

FerroptosisUrinary Bladder NeoplasmsHeat-Shock ProteinsHumansMolecular Chaperonesp38 Mitogen-Activated Protein KinasesPiperazinesRNA, CircularerastinHeat-Shock ProteinsHSBP1 protein, humanHSPB1 protein, humanMolecular Chaperonesp38 Mitogen-Activated Protein KinasesPiperazinesRNA, Circular

Identifiers

PMID35945269
OpenAlexW4290729766

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.