Evidence map›Paper›PMID 35944838›Full record

ArticleGenomics, proteomics & bioinformatics2022

Single-cell Sequencing Reveals Clearance of Blastula Chromosomal Mosaicism in In Vitro Fertilization Babies.

Yuan Gao, Jinning Zhang, Zhenyu Liu, Shuyue Qi, Xinmeng Guo, Hui Wang, Yanfei Cheng, Shuang Tian, Minyue Ma, Hongmei Peng and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Genomics, proteomics & bioinformatics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed, 1 pooled it
3.0field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 1 synthesis or guideline pooled it, 8 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Article
  4. Review
  5. Review
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 2 institutions in 2 countries.

Yuan GaoBiomedical Pioneering Innovation Center, School of Life Sciences, Peking University, Beijing 100871, China; Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China; Beijing Advanced Innovation Center for Genomics, MOE Key Laboratory of Cell Proliferation and Differentiation, Beijing 100871, China.
Jinning ZhangDepartment of Gynaecology and Obstetrics, The First Medical Center of PLA General Hospital, Beijing 100853, China; Reproductive Medicine and Prenatal Diagnosis Center, The University of Hong Kong-Shenzhen Hospital, Shenzhen 518053, China.
Zhenyu LiuBiomedical Pioneering Innovation Center, School of Life Sciences, Peking University, Beijing 100871, China; Beijing Advanced Innovation Center for Genomics, MOE Key Laboratory of Cell Proliferation and Differentiation, Beijing 100871, China.
Shuyue QiBiomedical Pioneering Innovation Center, School of Life Sciences, Peking University, Beijing 100871, China; Beijing Advanced Innovation Center for Genomics, MOE Key Laboratory of Cell Proliferation and Differentiation, Beijing 100871, China.
Xinmeng GuoDepartment of Gynaecology and Obstetrics, The First Medical Center of PLA General Hospital, Beijing 100853, China; Reproductive Medicine and Prenatal Diagnosis Center, The University of Hong Kong-Shenzhen Hospital, Shenzhen 518053, China.
Hui WangDepartment of Gynaecology and Obstetrics, The First Medical Center of PLA General Hospital, Beijing 100853, China; Reproductive Medicine and Prenatal Diagnosis Center, The University of Hong Kong-Shenzhen Hospital, Shenzhen 518053, China.
Yanfei ChengDepartment of Gynaecology and Obstetrics, The First Medical Center of PLA General Hospital, Beijing 100853, China; Reproductive Medicine and Prenatal Diagnosis Center, The University of Hong Kong-Shenzhen Hospital, Shenzhen 518053, China.
Shuang TianDepartment of Gynaecology and Obstetrics, The First Medical Center of PLA General Hospital, Beijing 100853, China; Reproductive Medicine and Prenatal Diagnosis Center, The University of Hong Kong-Shenzhen Hospital, Shenzhen 518053, China.
Minyue MaDepartment of Gynaecology and Obstetrics, The First Medical Center of PLA General Hospital, Beijing 100853, China; Reproductive Medicine and Prenatal Diagnosis Center, The University of Hong Kong-Shenzhen Hospital, Shenzhen 518053, China.
Hongmei PengDepartment of Gynaecology and Obstetrics, The First Medical Center of PLA General Hospital, Beijing 100853, China; Reproductive Medicine and Prenatal Diagnosis Center, The University of Hong Kong-Shenzhen Hospital, Shenzhen 518053, China.
Lu WenBiomedical Pioneering Innovation Center, School of Life Sciences, Peking University, Beijing 100871, China; Beijing Advanced Innovation Center for Genomics, MOE Key Laboratory of Cell Proliferation and Differentiation, Beijing 100871, China.
Fuchou TangBiomedical Pioneering Innovation Center, School of Life Sciences, Peking University, Beijing 100871, China; Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China; Beijing Advanced Innovation Center for Genomics, MOE Key Laboratory of Cell Proliferation and Differentiation, Beijing 100871, China. Electronic address: tangfuchou@pku.edu.cn.
Yuanqing YaoDepartment of Gynaecology and Obstetrics, The First Medical Center of PLA General Hospital, Beijing 100853, China; Reproductive Medicine and Prenatal Diagnosis Center, The University of Hong Kong-Shenzhen Hospital, Shenzhen 518053, China. Electronic address: yaoyq@hku-szh.org.
Chinese PLA General Hospital · CNPeking University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Although chromosomal mosaic embryos detected by trophectoderm (TE) biopsy offer healthy embryos available for transfer, high-resolution postnatal karyotyping and chromosome testing of the transferred embryos are insufficient. Here, we applied single-cell multi-omics sequencing for seven infants with blastula chromosomal mosaicism detected by TE biopsy. The chromosome ploidy was examined by single-cell genome analysis, with the cellular identity being identified by single-cell transcriptome analysis. A total of 1616 peripheral leukocytes from seven infants with embryonic chromosomal mosaicism and three control ones with euploid TE biopsy were analyzed. A small number of blood cells showed copy number alterations (CNAs) on seemingly random locations at a frequency of 0%-2.5% per infant. However, none of the cells showed CNAs that were the same as those of the corresponding TE biopsies. The blastula chromosomal mosaicism may be fully self-corrected, probably through the selective loss of the aneuploid cells during development, and the transferred embryos can be born as euploid infants without mosaic CNAs corresponding to the TE biopsies. The results provide a new reference for the evaluations of transferring chromosomal mosaic embryos in certain situations.

Indexed as

Preimplantation DiagnosisBlastocystBlastulaFemaleFertilization in VitroHigh-Throughput Nucleotide SequencingHumansMosaicismPregnancyMosaic embryo transferMosaicismNext-generation sequencingPre-implantation genetic testing for aneuploidySingle-cell multi-omics sequencing

Identifiers

PMID35944838
PMCPMC10225483
OpenAlexW4290052856

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.