ArticleMedical principles and practice : international journal of the Kuwait University, Health Science Centre2022
Multiplex gyrB PCR Assay for Identification of Acinetobacter baumannii Is Validated by Whole Genome Sequence-Based Assays.
Article in Medical principles and practice : international journal of the Kuwait University, Health Science Centre, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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5 citing papers in PubMed, 7 citations in OpenAlex.
- Integrating machine learning and artificial intelligence in the management of Acinetobacter infections: a narrative review.Infection · 2026Review
- Identification, resistance mechanisms, and innovative therapeutic approaches againstFrontiers in microbiology · 2026Review
- Pre-trained ChatGPT for report generation in automated microbial identification and antibiotic susceptibility testing systems.Scientific reports · 2025Article
- The intricacies ofFrontiers in microbiology · 2025Review
- Comparative pangenomic analysis of Campylobacter fetus isolated from Spanish bulls and other mammalian species.Scientific reports · 2024Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveA multiplex gyrB PCR assay has been used to diagnose Acinetobacter baumannii. However, this assay has not been validated against the gold standard DNA-DNA hybridization assay, which is a laborious method. DNA-DNA hybridization assay is now replaced by whole genome sequence (WGS)-based methods. Two such methods are a k-mer-based search of sequence reads using the Kraken 2 program and average nucleotide identity (ANI). The objective was to validate the gyrB PCR assay with WGS-based methods. SUBJECTS AND
methodsWe cultured 270 sequential A. baumannii isolates from the rectal swabs of 32 adult patients. The identity of the isolates was determined by gyrB PCR. The sequences of 269 isolates were determined by Illumina sequencing and the taxonomy was inferred by the Kraken 2 program and ANI.
resultsAll the 269 isolates were confirmed as A. baumannii by Kraken 2 and ANI.
conclusionThe gyrB PCR assay is now validated for easy identification of A. baumannii in comparison with gold standard WGS-based assays.
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