ArticleClinical infectious diseases : an official publication of the Infectious Diseases Society of America2023
Targeted Versus Shotgun Metagenomic Sequencing-based Detection of Microorganisms in Sonicate Fluid for Periprosthetic Joint Infection Diagnosis.
Article in Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers, 1 of them a synthesis that pooled it.
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Who cites it
49 citing papers in PubMed, 1 synthesis or guideline pooled it, 74 citations in OpenAlex.
- Comparison of microbial detection rates in microbial culture methods versus next-generation sequencing in patients with prosthetic joint infection: a systematic review and meta-analysis.Journal of orthopaedic surgery and research · 2023Pooled it
- 16S rRNA Bacterial Identification in Evaluation of Orthopedic Infections and its Limited Role in Antimicrobial Stewardship.Open forum infectious diseases · 2026Article
- Diagnostic and Clinical Value of Targeted Next-Generation Sequencing for Pediatric Respiratory Infections in Northern China.The clinical respiratory journal · 2026Article
- Article
- Real-life impact of clinical metagenomics in the intensive care unit: a multicenter retrospective study in greater paris area hospitals.Critical care (London, England) · 2026Article
- Rapid molecular culture versus conventional culture for periprosthetic joint infection: diagnostic performance and clinical relevance.Journal of bone and joint infection · 2026Article
- Development of a multiplex ddPCR assay for simultaneous absolute quantification of bacterial, fungal, and human DNA.PloS one · 2026Article
- From serum inflammatory markers to fluid, tissue, and molecular assays: current advances in the laboratory diagnosis of bone and joint infections.Frontiers in cellular and infection microbiology · 2026Review
- Toxoplasma gondii infection diagnosed by tNGS in a patient with acute lymphoblastic leukemia: a case report.BMC infectious diseases · 2025Article
- Beyond Cultures: The Evolving Role of Molecular Diagnostics, Synovial Biomarkers and Artificial Intelligence in the Diagnosis of Prosthetic Joint Infections.Journal of clinical medicine · 2025Review
- BioFire in osteoarticular Infections: Rapid syndromic testing for early and accurate diagnosis - A narrative review.Journal of clinical orthopaedics and trauma · 2025Review
- UMPlex™: a targeted next-generation sequencing primer design workflow.Virology journal · 2025Article
- Targeted next-generation sequencing - a promising approach in the diagnosis of Mycobacterium tuberculosis and drug resistance.Infection · 2025Article
- Targeted next-generation sequencing enhances precision and rapid detection in healthcare-associated infection Surveillance: Unveiling multidrug-resistant colonization in ICUs.New microbes and new infections · 2025Article
- Preoperative Synovial Fluid Cultures, and Biopsy Cultures After Dry Tap Aspiration, Are Valuable in Diagnosing a Periprosthetic Joint Infection: A Retrospective Study.Microorganisms · 2025Article
- MASP1 modulation as a novel therapeutic target in severe pediatric pertussis: insights from a multi-omics approach.Infection and immunity · 2025Article
- Diagnosis of acute Q fever using targeted next‑generation sequencing: A case report.Experimental and therapeutic medicine · 2025Article
- Combination of laboratory automation and next-generation sequencing for the diagnosis of osteoarticular infections: a technical note.Frontiers in surgery · 2025Article
- CD4Frontiers in cellular and infection microbiology · 2025Article
- Article
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Authors and funding
7 authors at 2 institutions in 3 countries.
Funding
Abstract
backgroundNext-generation sequencing (NGS) is increasingly used for periprosthetic joint infection (PJI) diagnosis, but its clinical utility is poorly defined. Shotgun metagenomic sequencing (sNGS) has been reported to identify PJI pathogens undetected by culture in sonicate fluid. However, sNGS is complex and costly. Here, 16S ribosomal RNA (rRNA) gene-based targeted metagenomic sequencing (tNGS) was compared to sNGS of sonicate fluid for microbial detection and identification in patients with total hip arthroplasty (THA) and total knee arthroplasty (TKA) failure.
methodsA convenience sample of sonicate fluids derived from patients who had undergone THA or TKA removal, enriched with culture negative PJI cases, was tested. Samples had been previously tested by sNGS. For tNGS, samples were extracted, amplified by polymerase chain reaction targeting the V1 to V3 regions of the 16S rRNA gene, and sequenced on an Illumina MiSeq.
resultsA total of 395 sonicate fluids, including 208 from subjects with PJI, were studied. Compared with sonicate fluid culture, tNGS had higher positive percent agreement (72.1 vs 52.9%, P < .001), detecting potential pathogens in 48.0% of culture-negative PJIs. There was no difference between the positive percent agreement of tNGS (72.1%) and sNGS (73.1%, P = .83).
conclusions16S rRNA gene-based tNGS is a potential diagnostic tool for PJI pathogen identification in sonicate fluid from failed THAs and TKAs in culture-negative cases, with similar performance characteristics to sNGS.
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