Evidence map›Paper›PMID 35943692›Full record

ArticleSleep & breathing = Schlaf & Atmung2023

The role of PPARγ in intermittent hypoxia-related human umbilical vein endothelial cell injury.

Lian Ning-Fang, Jin Yong-Xu, Chen Jia, Wang Cai-Yun, Huang Jie-Feng, Lin Qi-Chang

Abstract read
PubMed Publisher
In one paragraph

Article in Sleep & breathing = Schlaf & Atmung, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Lian Ning-FangDepartment of Respiratory and Critical Care Medicine, Fujian Provincial Sleep-Disordered Breathing Clinic Center; Institute of Respiratory Disease, the First Affiliated Hospital of Fujian Medical University, Fujian Medical University, No 20, Chazhong road, Taijiang district, Fujian, 350005, Fuzhou, China.
Jin Yong-XuDepartment of Respiratory and Critical Care Medicine, Fujian Provincial Sleep-Disordered Breathing Clinic Center; Institute of Respiratory Disease, the First Affiliated Hospital of Fujian Medical University, Fujian Medical University, No 20, Chazhong road, Taijiang district, Fujian, 350005, Fuzhou, China.
Chen JiaDepartment of Respiratory and Critical Care Medicine, Fujian Provincial Sleep-Disordered Breathing Clinic Center; Institute of Respiratory Disease, the First Affiliated Hospital of Fujian Medical University, Fujian Medical University, No 20, Chazhong road, Taijiang district, Fujian, 350005, Fuzhou, China.
Wang Cai-YunDepartment of Respiratory and Critical Care Medicine, Fujian Provincial Sleep-Disordered Breathing Clinic Center; Institute of Respiratory Disease, the First Affiliated Hospital of Fujian Medical University, Fujian Medical University, No 20, Chazhong road, Taijiang district, Fujian, 350005, Fuzhou, China.
Huang Jie-FengDepartment of Respiratory and Critical Care Medicine, Fujian Provincial Sleep-Disordered Breathing Clinic Center; Institute of Respiratory Disease, the First Affiliated Hospital of Fujian Medical University, Fujian Medical University, No 20, Chazhong road, Taijiang district, Fujian, 350005, Fuzhou, China.
Lin Qi-ChangDepartment of Respiratory and Critical Care Medicine, Fujian Provincial Sleep-Disordered Breathing Clinic Center; Institute of Respiratory Disease, the First Affiliated Hospital of Fujian Medical University, Fujian Medical University, No 20, Chazhong road, Taijiang district, Fujian, 350005, Fuzhou, China. linqc_fy@126.com.ORCID 0000-0003-3081-8084
Fujian Medical University · CN

Funding

Fujian Province Finance Project BPB-LNF2021Joint Funds for the innovation of science and Technology, Fujian Province 2019Y9116
6 · The paper itself

Abstract

purposePatients with obstructive sleep apnoea (OSA) have a high incidence of vascular endothelial injury. The most important pathophysiological feature of OSA is chronic intermittent hypoxia (CIH). This study aimed to investigate the mechanisms of CIH-related vascular endothelial injury.

methodsIH exposure was applied to human umbilical vein endothelial cells (HUVECs). After modeling, cell viability, the expression levels of peroxisome proliferator activated receptor γ (PPARγ), apoptosis-associated proteins and mitochondrial division fusion proteins, and the levels of reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) were assessed via Cell Counting Kit-8 (CCK-8), western blotting, fluorescent microscope, and flow cytometry, respectively. Rosiglitazone (PPARγ agonist), tempo (the mitochondrial-specific antioxidant), and tempo combined with PPARγ interfering RNA were used to treat HUVECs, respectively.

resultsAfter IH exposure, cell viability and levels of MMP decreased, cell apoptosis and ROS levels increased, and the expression levels of PPARγ decreased. Both tempo and rosiglitazone pretreatment ameliorated cell apoptosis and improved cell viability. In addition, mitochondrial function became better after tempo pretreatment. PPARγ interference reversed the protective effects of tempo on IH-related mitochondrial function injury and cell injury.

conclusionsPPARγ regulated the apoptosis and cell viability of IH-treated HUVECs by altering mitochondrial function. This finding clarifies the mechanism of CIH-related vascular endothelial injury.

Indexed as

PPAR gammaSleep Apnea, ObstructiveApoptosisHumansHuman Umbilical Vein Endothelial CellsHypoxiaReactive Oxygen SpeciesRosiglitazonePPAR gammaReactive Oxygen SpeciesRosiglitazoneEndothelial dysfunctionIntermittent hypoxiaObstructive sleep apneaPPARγ

Identifiers

PMID35943692
OpenAlexW4292451987

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.