Evidence map›Paper›PMID 35943638›Full record

ArticleMarine biotechnology (New York, N.Y.)2022

Improved Growth and High Inheritance of Melanocortin-4 Receptor (mc4r) Mutation in CRISPR/Cas-9 Gene-Edited Channel Catfish, Ictalurus punctatus.

Michael Coogan, Veronica Alston, Baofeng Su, Karim Khalil, Ahmed Elaswad, Mohd Khan, Andrew Johnson, De Xing, Shangjia Li, Jinhai Wang and 11 more

Abstract read
PubMed Publisher
In one paragraph

Article in Marine biotechnology (New York, N.Y.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.8field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 24 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. CRISPR-Cas9 in the Tailoring of Genetically Engineered Animals.Current issues in molecular biology · 2025
    Review
  5. Review
  6. Article
  7. Review
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors at 5 institutions in 4 countries.

Michael CooganAquaculture and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA. mpcoogan92@gmail.com.ORCID http://orcid.org/0000-0002-5438-6530
Veronica AlstonAquaculture and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA.
Baofeng SuAquaculture and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA.
Karim KhalilAquaculture and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA.
Ahmed ElaswadAquaculture and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA.
Mohd KhanAquaculture and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA.
Andrew JohnsonAquaculture and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA.
De XingAquaculture and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA.
Shangjia LiAquaculture and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA.
Jinhai WangAquaculture and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA.
Rhoda M C SimoraAquaculture and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA.
Cuiyu LuAquaculture and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA.
Patrick Page-McCawDepartment of Molecular and Integrative Physiology, Vanderbilt University, Nashville, TN, 37203-5721, USA.
Wenbiao ChenDepartment of Molecular and Integrative Physiology, Vanderbilt University, Nashville, TN, 37203-5721, USA.
Max MichelDepartment of Molecular and Integrative Physiology, Vanderbilt University, Nashville, TN, 37203-5721, USA.
Wenwen WangAquaculture and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA.
Darshika HettiarachchiAquaculture and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA.
Tasnuba HasinAquaculture and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA.
Ian A E ButtsAquaculture and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA.
Roger D ConeDepartment of Molecular and Integrative Physiology, Vanderbilt University, Nashville, TN, 37203-5721, USA.
Rex A DunhamAquaculture and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA.
Auburn University · USVanderbilt University · USBangladesh Agricultural University · BDSuez Canal University · EGUniversity of the Philippines Visayas · PH

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Effects of CRISPR/Cas9 knockout of the melanocortin-4 receptor (mc4r) gene in channel catfish, Ictalurus punctatus, were investigated. Three sgRNAs targeting the channel catfish mc4r gene in conjunction with Cas9 protein were microinjected in embryos and mutation rate, inheritance, and growth were studied. Efficient mutagenesis was achieved as demonstrated by PCR, Surveyor® assay, and DNA sequencing. An overall mutation rate of 33% and 33% homozygosity/bi-allelism was achieved in 2017. Approximately 71% of progeny inherited the mutation. Growth was generally higher in MC4R mutants than controls (CNTRL) at all life stages and in both pond and tank environments. There was a positive relationship between zygosity and growth, with F

Indexed as

IctaluridaeAnimalsCRISPR-Associated Protein 9CRISPR-Cas SystemsGene EditingHumansMutationReceptor, Melanocortin, Type 4CRISPR-Associated Protein 9MC4R protein, humanReceptor, Melanocortin, Type 4CRISPR/Cas9GrowthIctalurus punctatusmc4rMelanocortin-4 receptor

Identifiers

PMID35943638
OpenAlexW4290772051

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.