Top-Down Proteomics by Capillary Zone Electrophoresis-Tandem Mass Spectrometry for Large-Scale Characterization of Proteoforms in Complex Samples.

Elijah N McCool, Rachele A Lubeckyj, Daoyang Chen, Liangliang Sun

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

2.5field-weighted citation impact, top 12% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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1 citing paper in PubMed, 4 citations in OpenAlex.

Prediction, Discovery, and Characterization of Plant- and Food-Derived Health-Beneficial Bioactive Peptides.Nutrients · 2022
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4 · The record

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5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Elijah N McCoolDepartment of Chemistry, Michigan State University, East Lansing, MI, USA.

Rachele A LubeckyjDepartment of Chemistry, Michigan State University, East Lansing, MI, USA.

Daoyang ChenDepartment of Chemistry, Michigan State University, East Lansing, MI, USA.

Liangliang SunDepartment of Chemistry, Michigan State University, East Lansing, MI, USA. lsun@chemistry.msu.edu.

Michigan State University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Capillary zone electrophoresis (CZE) is a fundamentally simple and highly efficient separation technique based on differences in electrophoretic mobilities of analytes. CZE-mass spectrometry (MS) has become an important analytical tool in top-down proteomics which aims to delineate proteoforms in cells comprehensively, because of the improvement of capillary coatings, sample stacking methods, and CE-MS interfaces. Here, we present a CZE-MS/MS-based top-down proteomics procedure for the characterization of a standard protein mixture and an Escherichia coli (E. coli) cell lysate using linear polyacrylamide-coated capillaries, a dynamic pH junction sample stacking method, a commercialized electro-kinetically pumped sheath flow CE-MS interface and an Orbitrap mass spectrometer. CZE-MS/MS can identify hundreds of proteoforms routinely from the E. coli sample with a 1% proteoform-level false discovery rate (FDR).

Indexed as

Escherichia coli ProteinsProteomicsElectrophoresis, CapillaryEscherichia coliTandem Mass SpectrometryEscherichia coli ProteinsAnalytical methodCapillary zone electrophoresisCE-MS interfaceDynamic pH junctionLinear polyacrylamide coatingMass spectrometryProteoformSeparationTop-down proteomics

Identifiers

PMID35941482

DOI10.1038/nmeth1019

OpenAlexW4294920539

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.