ArticleParasitology research2022
Ultrastructure Expansion Microscopy (U-ExM) in Trypanosoma cruzi: localization of tubulin isoforms and isotypes.
Article in Parasitology research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Ultrastructural expansion microscopy reveals unexpected levels of glycosome heterogeneity in African trypanosomes.Journal of microscopy · 2026Article
- Expansion Microscopy in Euglenozoa.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Accelerated protein retention expansion microscopy using microwave radiation.Cell reports methods · 2024Article
- Accelerated protein retention expansion microscopy using microwave radiation.bioRxiv : the preprint server for biology · 2024Article
- Continuous endosomes form functional subdomains and orchestrate rapid membrane trafficking in trypanosomes.eLife · 2024Article
- New Insights into the Role of thePathogens (Basel, Switzerland) · 2023Article
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1 author.
Funding
Abstract
Ultrastructure Expansion Microscopy (U-ExM) is a recently developed technique that enables the increase of the spatial resolution within a cell or a tissue for microscopic imaging by physically expanding the sample. For the first time, I report a detailed protocol validating the use of U-ExM in Trypanosoma cruzi and quantifying the expansion factors of different subcellular compartments. I was able to determine the localization patterns of different tubulin isoforms, such as α-tubulin and β-tubulin. Also, I immunolocalized acetylated and tyrosinated α-tubulin isotypes in epimastigotes and use mitochondrial cell-permeable dyes to identify this organelle. Finally, U-ExM was also performed in trypomastigotes and amastigotes validating this technique in all life cycle stages of T. cruzi.
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Registered trials
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