ArticleNature communications2022
A quantitative analysis of various patterns applied in lattice light sheet microscopy.
Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 32 citations in OpenAlex.
- A multimodal adaptive optical microscope for in vivo imaging from molecules to organisms.Nature methods · 2026Article
- Polarization-engineered aberration-resilient light sheet microscopy.bioRxiv : the preprint server for biology · 2026Article
- Single-Objective Lattice Light Sheet Microscopy with Microfluidics for Single-Molecule Super-Resolution Imaging of Mammalian Cells.ACS photonics · 2026Article
- Synthetic ZFTA fusions pinpoint disordered protein domain acquisition as a mechanism of brain tumorigenesis.Nature cell biology · 2025Article
- Advancing Multicolor Super-Resolution Volume Imaging: Illuminating Complex Cellular Dynamics.JACS Au · 2025Review
- A Multimodal Adaptive Optical Microscope ForbioRxiv : the preprint server for biology · 2025Article
- Comparative prospects of imaging methods for whole-brain mammalian connectomics.Cell reports methods · 2025Review
- Optical sectioning methods in three-dimensional bioimaging.Light, science & applications · 2025Review
- Model based optimization for refractive index mismatched light sheet imaging.Optics express · 2024Article
- Applications of Lightsheet Fluorescence Microscopy by High Numerical Aperture Detection Lens.The journal of physical chemistry. B · 2024Review
- A fluorogenic complementation tool kit for interrogating lipid droplet-organelle interaction.The Journal of cell biology · 2024Article
- Posterior approach to correct for focal plane offsets in lattice light-sheet structured illumination microscopy.Journal of biomedical optics · 2024Article
- Correlative single molecule lattice light sheet imaging reveals the dynamic relationship between nucleosomes and the local chromatin environment.Nature communications · 2024Article
- Intelligent Beam Optimization for Light-Sheet Fluorescence Microscopy through Deep Learning.Intelligent computing (Washington, D.C.) · 2024Article
- Protein and lipid expansion microscopy with trypsin and tyramide signal amplification for 3D imaging.Scientific reports · 2023Article
- Enhancing Light-Sheet Fluorescence Microscopy Illumination Beams through Deep Design Optimization.bioRxiv : the preprint server for biology · 2023Article
- A fluorogenic complementation tool kit for interrogating lipid droplet-organelle interaction.bioRxiv : the preprint server for biology · 2023Article
- Bridging live-cell imaging and next-generation cancer treatment.Nature reviews. Cancer · 2023Review
- Article
- Formation of multiple complex light structures simultaneously in 3D volume using a single binary phase mask.Scientific reports · 2023Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Light sheet microscopes reduce phototoxicity and background and improve imaging speed compared to widefield and confocal microscopes. However, when equipped with Gaussian beams, the axial resolving power of a light sheet microscope and the observable field of view are inversely related. Light sheets based on dithered optical lattices improve axial resolution and beam uniformity compared Gaussian beams by using axially structured illumination patterns. However, these advantages come at the expense of an increased total illumination to the specimen and a decreased axial confinement of the illumination pattern. Using simulations and experimental measurements in fixed and live cells, we quantify the differences between Gaussian and lattice light sheets on beam uniformity, axial resolution, lateral resolution, and photobleaching. We demonstrate how different optical lattice illumination patterns can be tuned to prioritize either axial resolution or optical sectioning. Finally, we introduce an approach to spectrally fuse sequential acquisitions of different lattice light sheet patterns with complementary optical properties to achieve both high resolution and low background images.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.