ArticleJournal of advanced research2023
The clock component OsLUX regulates rice heading through recruiting OsELF3-1 and OsELF4s to repress Hd1 and Ghd7.
Article in Journal of advanced research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 25 citations in OpenAlex.
- Identification of Gene Regulatory Network for Nitrogen-Promoted Tiller Regrowth in Perennial Rice.Rice (New York, N.Y.) · 2026Article
- The blue light receptor ZmFKF1a recruits ZmGI1 to the nucleus to accelerate shoot apex development and flowering in maize.The Plant cell · 2025Article
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- Transcriptional activation and repression in the plant circadian clock: Revisiting core oscillator feedback loops and output pathways.Plant communications · 2025Review
- Allelic Variation of Hd17 for Rice Heading Date is Caused by Natural Selection.Rice (New York, N.Y.) · 2025Article
- The integration of quantile regression with 3VmrMLM identifies more QTNs and QTN-by-environment interactions using SNP- and haplotype-based markers.Plant communications · 2025Article
- A Series of Novel Alleles ofPlants (Basel, Switzerland) · 2025Article
- OsBBX2 Delays Flowering by RepressingPlants (Basel, Switzerland) · 2024Article
- Candidate gene analysis of rice grain shape based on genome-wide association study.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Research Progress on Photoperiod Gene Regulation of Heading Date in Rice.Current issues in molecular biology · 2024Review
- OsCOL5 suppresses heading through modulation of Ghd7 and Ehd2, enhancing rice yield.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Unlocking Nature's Clock: CRISPR Technology in Flowering Time Engineering.Plants (Basel, Switzerland) · 2023Review
- PnMYB4 negatively modulates saponin biosynthesis inHorticulture research · 2023Article
- Two interacting basic helix-loop-helix transcription factors control flowering time in rice.Plant physiology · 2023Article
- Article
- Photoperiod Genes Contribute to Daylength-Sensing and Breeding in Rice.Plants (Basel, Switzerland) · 2023Article
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19 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
introductionCircadian clocks coordinate internal physiology and external environmental factors to regulate cereals flowering, which is critical for reproductive growth and optimal yield determination.
objectivesIn this study, we aimed to confirm the role of OsLUX in flowering time regulation in rice. Further research illustrates how the OsELF4s-OsELF3-1-OsLUX complex directly regulates flowering-related genes to mediate rice heading.
methodsWe identified a circadian gene OsLUX by the MutMap method. The transcription levels of flowering-related genes were evaluated in WT and oslux mutants. OsLUX forms OsEC (OsELF4s-OsELF3-1-OsLUX) complex were supported by yeast two-hybrid, pull down, BiFC, and luciferase complementation assays (LCA). The EMSA, Chip-qPCR, luciferase luminescence images, and relative LUC activity assays were performed to examine the targeted regulation of flowering genes by the OsEC (OsELF4s-OsELF3-1-OsLUX) complex.
resultsThe circadian gene OsLUX encodes an MYB family transcription factor that functions as a vital circadian clock regulator and controls rice heading. Defect in OsLUX causes an extremely late heading phenotype under natural long-day and short-day conditions, and the function was further confirmed through genetic complementation, overexpression, and CRISPR/Cas9 knockout. OsLUX forms the OsEC (OsELF4s-OsELF3-1-OsLUX) complex by recruiting OsELF3-1 and OsELF4s, which were required to regulate rice heading. OsELF3-1 contributes to the translocation of OsLUX to the nucleus, and a compromised flowering phenotype results upon mutation of any component of the OsEC complex. The OsEC complex directly represses Hd1 and Ghd7 expression via binding to their promoter's LBS (LUX binding site) element.
conclusionOur findings show that the circadian gene OsLUX regulates rice heading by directly regulating rhythm oscillation and core flowering-time-related genes. We uncovered a mechanism by which the OsEC target suppresses the expression of Hd1 and Ghd7 directly to modulate photoperiodic flowering in rice. The OsEC (OsELF4s-OsELF3-1-OsLUX)-Hd1/Ghd7 regulatory module provides the genetic targets for crop improvement.
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