ArticleLife sciences2022
Expression of CYP450 enzymes in human fetal membranes and its implications in xenobiotic metabolism during pregnancy.
Article in Life sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 23 citations in OpenAlex.
- Anti-inflammatory and anti-rejection effects of herbal medicine ingredients in organ transplantation: a systematic review and meta-analysis.Frontiers in immunology · 2025Pooled it
- Epigenetic and immunological alterations in umbilical cord blood of overweight/obese women with gestational diabetes mellitus: insights into DNA methylation signatures and immune cell dysregulation.BMC pregnancy and childbirth · 2026Article
- Acetaminophen and pregnancy: common exposure, potential placental impact.Journal of animal science · 2026Review
- Endocrine Disruptors at the Fetomaternal Interface: Insight From PBDE Studies and the Utility of Novel Approach Methods.Endocrinology · 2026Review
- Fetal response to maternal exposures of environmental chemicals: Utility of a four-cell human feto-maternal interface organ-on-chip.Chemico-biological interactions · 2025Article
- A call to standardize the nomenclature of human fetal membrane at the feto-maternal interface.Placenta · 2025Review
- Exofection by exosomes: A transient functional cargo transfer.Extracellular vesicle · 2025Article
- Microfluidic device successfully replaces traditional models of pregnancy associated drug pharmacokinetic studies.Pregnancy (Hoboken, N.J.) · 2025Article
- Determining Sex-Specific Gene Expression Differences in Human Chorion Trophoblast Cells.International journal of molecular sciences · 2025Article
- Gestational diabetes increases preterm membrane rupture risk through elevated hydroxydesmethylpiperine sulfate levels.Frontiers in endocrinology · 2025Observational
- Strategy advancements in placental pharmacokinetics: fromFrontiers in pharmacology · 2025Review
- PGRMC2 and HLA-G regulate immune homeostasis in a microphysiological model of human maternal-fetal membrane interface.Communications biology · 2024Article
- High-Throughput 3D-Printed Model of the Feto-Maternal Interface for the Discovery and Development of Preterm Birth Therapies.ACS applied materials & interfaces · 2024Article
- Decoding the molecular pathways governing trophoblast migration and placental development; a literature review.Frontiers in endocrinology · 2024Review
- Placenta: an old organ with new functions.Frontiers in immunology · 2024Review
- Augmentation of Pectoral Fin Teratogenicity by Thalidomide in Human Cytochrome P450 3A-Expressing Zebrafish.Pharmaceuticals (Basel, Switzerland) · 2023Article
- Microfluidic technology and simulation models in studying pharmacokinetics during pregnancy.Frontiers in pharmacology · 2023Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
Abstract
backgroundEnvironmental exposure to toxicants is a major risk factor for spontaneous preterm birth (PTB, <37 weeks). Toxicants and drugs administered to patients are metabolized primarily by the cytochrome P450 (CYP450) system. Along with the adult and fetal liver, the placenta, a critical feto-maternal interface organ, expresses CYP450 enzymes that metabolize these xenobiotics. However, the contribution of the fetal membranes, another tissue of the feto-maternal interface, to the expression of CYP450 enzymes and the detoxification of xenobiotics remains unknown.
aimsThis study characterized CYP450 expression and determined the functional activity of CYP450 enzymes in fetal membranes. MAIN
methodsRNA sequencing (RNA-Seq) of placental and fetal membrane tissues and cells was done. Differential expressions of CYP450 genes were compared and validated via reverse transcription-quantitative polymerase chain reaction (RT-qPCR) between the two tissues. The functional activity of major CYP450 enzymes was determined using a fluorophore-based enzymatic assay in the presence and absence of their corresponding inhibitors. KEY
findingsWith the exception of genes that regulate cholesterol metabolism, the expression profile of CYP450 genes was similar between placental and fetal membranes tissues/cells. RT-qPCR analysis confirmed these findings with significant levels of mRNA for major CYP450 genes being detectable in amnion epithelial cells (AECs) and chorion trophoblasts cells (CTCs). Biochemical analyses revealed significant CYP450 enzymatic activities that were sensitive to specific inhibitors for both AECs and CTCs, suggesting that the genes were expressed as functional enzymes. SIGNIFICANCE: This is the first study to determine global expression of CYP450 enzymes in fetal membranes which may play a role in xenobiotic metabolism during pregnancy. Given that many women are exposed to environmental toxins or require medications during pregnancy, a better understanding of their role in metabolism is required to develop safer therapeutics and prevent adverse outcomes.
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