ArticleParasitology research2022
Untangling the actual infection status: detection of avian haemosporidian parasites of three Malagasy bird species using microscopy, multiplex PCR, and nested PCR methods.
Article in Parasitology research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 20 citations in OpenAlex.
- Review of Genetic Information of Morphologically Described Haemoproteus Parasites Recorded in GenBank and MalAvi Databases.Integrative zoology · 2026Review
- Host Traits and Avian Malaria Parasites: Understanding the Intrinsic Connections.Integrative zoology · 2026Article
- Heterogeneous distribution of avian malaria parasites across mosquito species: disproportionate involvement of Culex restuans in the Midwestern United States.Malaria journal · 2026Article
- Avian haemosporidian parasites in the African houbara: Species diversity and impact on blood parameters.Parasitology research · 2026Article
- Molecular detection of avian parasites in Australian mosquitoes (Culicidae).Journal of medical entomology · 2025Article
- Hemoparasites in Wild Birds: A Systematic Review of Their Ecology and Clinical Implications.Animals : an open access journal from MDPI · 2025Review
- The Study onInsects · 2025Article
- Avian Haemosporidian Parasites in Three Wild Columbids from Germany.Microorganisms · 2025Article
- Raising the bar: genus-specific nested PCR improves detection and lineage identification of avian haemosporidian parasites.Frontiers in cellular and infection microbiology · 2024Article
- Haemosporidian parasite infections of Malagasy Philepittidae and Nectariniidae are driven by phylogeny rather than ecology.Parasitology · 2023Article
- Mitochondrial genome amplification of avian haemosporidian parasites from single-infected wildlife samples using a novel nested PCR approach.Parasitology research · 2023Article
- Haemosporidian Parasites of White-Breasted Waterhens (Animals : an open access journal from MDPI · 2023Article
- Insights into the Biology ofMicroorganisms · 2023Review
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Authors and funding
4 authors at 2 institutions in 1 country.
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Abstract
The development of new molecular methods has significantly improved the detection and identification of avian haemosporidian parasites (Plasmodium, Haemoproteus and Leucocytozoon) compared to microscopic examination. Very large numbers of previously hidden Haemosporida species of a wide range of avian hosts have thus been discovered in the last two decades. However, test parameters of the various detection methods remain largely unevaluated. In this study, the merits of microscopy, multiplex PCR, and nested PCR were compared to identify the infection status of three Malagasy bird species. A total of 414 blood samples of Hypsipetes madagascariensis, Foudia omissa and F. madagascariensis, as well as 147 blood smears, were examined for haemosporidian infection. Thirty-four lineages of haemosporidian parasites could be identified, of which six have been detected for the first time. Microscopy, multiplex and nested PCR showed differences in detection rate, most likely due to low parasitemia of chronically infected birds. The combination of both PCR methods yielded the best results. In particular, detection of multiple infections could be greatly improved and will enable more precise prevalence estimates of individual haemosporidian species in wild birds in the future.
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