Evidence map›Paper›PMID 35934734›Full record

ArticleCurrent microbiology2022

Assessment of rSodC, rPknG, rNanH, and rSpaC as Antigens for Diagnostic Tools Against Caseous Lymphadenitis.

Ramon Mendes Dos Santos, Silvana Beutinger Marchioro, Marcos Borges Ribeiro, Maria da Conceição Aquino de Sá, Vítor Cordeiro Pereira, Antonio Pedro Froes de Farias, Roberto José Meyer Nascimento, Songelí Menezes Freire

Abstract read
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In one paragraph

Article in Current microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.2field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Ramon Mendes Dos SantosLaboratory of Immunology and Molecular Biology, Institute of Health Sciences, Federal University of Bahia, Av. Reitor Miguel Calmon, s/n-Canela, Salvador, Brazil.
Silvana Beutinger MarchioroLaboratory of Immunology and Molecular Biology, Institute of Health Sciences, Federal University of Bahia, Av. Reitor Miguel Calmon, s/n-Canela, Salvador, Brazil. silmarchioro@hotmail.com.
Marcos Borges RibeiroLaboratory of Immunology and Molecular Biology, Institute of Health Sciences, Federal University of Bahia, Av. Reitor Miguel Calmon, s/n-Canela, Salvador, Brazil.
Maria da Conceição Aquino de SáLaboratory of Immunology and Molecular Biology, Institute of Health Sciences, Federal University of Bahia, Av. Reitor Miguel Calmon, s/n-Canela, Salvador, Brazil.
Vítor Cordeiro PereiraLaboratory of Immunology and Molecular Biology, Institute of Health Sciences, Federal University of Bahia, Av. Reitor Miguel Calmon, s/n-Canela, Salvador, Brazil.
Antonio Pedro Froes de FariasLaboratory of Immunology and Molecular Biology, Institute of Health Sciences, Federal University of Bahia, Av. Reitor Miguel Calmon, s/n-Canela, Salvador, Brazil.
Roberto José Meyer NascimentoLaboratory of Immunology and Molecular Biology, Institute of Health Sciences, Federal University of Bahia, Av. Reitor Miguel Calmon, s/n-Canela, Salvador, Brazil.
Songelí Menezes FreireLaboratory of Immunology and Molecular Biology, Institute of Health Sciences, Federal University of Bahia, Av. Reitor Miguel Calmon, s/n-Canela, Salvador, Brazil.
Universidade Federal da Bahia · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Corynebacterium pseudotuberculosis is a bacillus that causes caseous lymphadenitis in small ruminants, leading to great losses to rural producers; thus, an efficient diagnosis is necessary for using disease control measures. This study aimed to evaluate the antigenic potential of four C. pseudotuberculosis recombinant proteins (rSodC, rPknG, rNanH, and rSpaC) against sera of goat and sheep experimentally infected with one of three different C. pseudotuberculosis strains. Goats were infected with CAP76 or CAP21 strain (n = 10), sheep with VD57 strain (n = 6), and a group of not-infected animals (goats and sheep) were kept as a healthy control (healthy n = 12). Sera were collected at 0, 14, 60, 90, 180, or 190 days after inoculation for antigenicity testing using Western blotting and enzyme-linked immunosorbent assay (ELISA) techniques. Cross-reactivity tests with recombinant proteins were performed in goat serum experimentally vaccinated with Nocardia sp. or Rhodococcus equi bacterin. The rSodC protein showed discriminatory antigenic reactivity with a statistically significant difference against three different C. pseudotuberculosis strains evaluated in goats and sheep samples, while rPknG showed statistical significance only against two C. pseudotuberculosis strains evaluated in goats. rSodC was proved to be a strong candidate as a tool for diagnosis of C. pseudotuberculosis infection, once it was able to recognize antibodies against all strains evaluated in goats and sheep.

Indexed as

Corynebacterium InfectionsGoat DiseasesLymphadenitisSheep DiseasesAnimalsGoatsRecombinant ProteinsSheepRecombinant Proteins

Identifiers

PMID35934734
OpenAlexW4290503889

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.