ArticlePlant methods2022
Establishment of Agrobacterium-mediated genetic transformation and application of CRISPR/Cas9 genome-editing system to Brassica rapa var. rapa.
Article in Plant methods, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 26 citations in OpenAlex.
- A transposon insertion in the promoter ofHorticulture research · 2026Article
- Application of Metabolomics in Defence Responses ofMetabolites · 2026Review
- WUSCHEL Transcription Factor: From Stem Cell Maintenance to Crop Improvement.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Study on Genetic Transformation System of Cabbage (Plants (Basel, Switzerland) · 2025Article
- Efficient and versatile rapeseed transformation for new breeding technologies.The Plant journal : for cell and molecular biology · 2025Article
- Haploid Production inBiology · 2025Review
- Improving transformation and regeneration efficiency in medicinal plants: insights from other recalcitrant species.Journal of experimental botany · 2025Review
- CRISPR-based gene editing in plants: Focus on reagents and their delivery tools.BioImpacts : BI · 2025Review
- Development of an optimized protocol for protoplast-to-plant regeneration of selected varieties of Brassica oleracea L.BMC plant biology · 2024Article
- Applications and Prospects of CRISPR/Cas9 Technology in the Breeding of Major Tropical Crops.Plants (Basel, Switzerland) · 2024Review
- Establishment and application of highly efficient regeneration, genetic transformation and genome editing system for cucurbitacins biosynthesis in Hemsleya chinensis.BMC plant biology · 2024Article
- Advances in and Perspectives on Transgenic Technology and CRISPR-Cas9 Gene Editing in Broccoli.Genes · 2024Review
- BrrTCP4b interacts with BrrTTG1 to suppress the development of trichomes inPlant diversity · 2024Article
- Guidelines for Performing CRISPR/Cas9 Genome Editing for Gene Validation and Trait Improvement in Crops.Plants (Basel, Switzerland) · 2023Review
- Optimization of Regeneration andPlants (Basel, Switzerland) · 2022Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundGenome editing is essential for crop molecular breeding. However, gene editing in turnip (Brassica rapa var. rapa) have not been reported owing to the very low transformation efficiency.
resultsIn this study, we established a transformation procedure involving chemical-inducible activation of the BrrWUSa gene, which resulted in high transformation frequencies of turnip. Estradiol-inducible BrrWUSa transgenic plants were fertile and showed no obvious developmental defects. Furthermore, we used CRISPR/Cas9 gene-editing technology to edit BrrTCP4b and generated 20 BrrTCP4b-edited seedlings with an increase in leaf trichome number.
conclusionThe results demonstrate that BrrWUSa improves the regeneration efficiency in turnip. The transformation procedure represents a promising strategy to improve genetic transformation and for functional characterization of genes in turnip.
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