Evidence map›Paper›PMID 35933037›Full record

ArticleJournal of invertebrate pathology2022

Comparative genomics reveals that metabolism underlies evolution of entomopathogenicity in bee-loving Ascosphaera spp. fungi.

J J Maccaro, J F Moreira Salgado, E Klinger, M P Argueta Guzmán, L Ngor, J E Stajich, Q S McFrederick

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of invertebrate pathology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.2field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

J J MaccaroDepartment of Entomology, University of California Riverside, Riverside, CA, USA.
J F Moreira SalgadoInstituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, RJ, Brazil; Department of Microbiology and Plant Pathology, University of California Riverside, Riverside, CA, USA.
E KlingerDepartment of Entomology, The Ohio State University, Columbus, OH, USA; USDA-ARS Pollinating Insect Biology Management Systematics Research Unit, Logan, UT, USA.
M P Argueta GuzmánDepartment of Entomology, University of California Riverside, Riverside, CA, USA.
L NgorDepartment of Entomology, University of California Riverside, Riverside, CA, USA.
J E StajichDepartment of Microbiology and Plant Pathology, University of California Riverside, Riverside, CA, USA. Electronic address: jason.stajich@ucr.edu.
Q S McFrederickDepartment of Entomology, University of California Riverside, Riverside, CA, USA. Electronic address: quinnmc@ucr.edu.
University of California, Riverside · USThe Ohio State University · US

Funding

Transmission Networks in Trait-Based CommunitiesR01GM122062 · NIGMS · CORNELL UNIVERSITY · PI ADLER, LYNN S., ELLNER, STEPHEN PAUL · 2016 to 2019
$1.9M
Acquisition of a Scalable Storage Cluster for Data Intensive NIH ResearchS10OD016290 · OD · UNIVERSITY OF CALIFORNIA RIVERSIDE · PI GIRKE, THOMAS · 2014 to 2014
$593k
NIGMS NIH HHS R01 GM122062NIH HHS S10 OD016290
6 · The paper itself

Abstract

Ascosphaera (Eurotiomycetes: Onygenales) is a diverse genus of fungi that is exclusively found in association with bee nests and comprises both saprophytic and entomopathogenic species. To date, most genomic analyses have been focused on the honeybee pathogen A. apis, and we lack a genomic understanding of how pathogenesis evolved more broadly in the genus. To address this gap we sequenced the genomes of the leaf-cutting bee pathogen A. aggregata as well as three commensal species: A. pollenicola, A. atra and A. acerosa. De novo annotation and comparison of the assembled genomes was carried out, including the previously published genome of A. apis. To identify candidate virulence genes in the pathogenic species, we performed secondary metabolite-oriented analyses and clustering of biosynthetic gene clusters (BGCs). Additionally, we captured single copy orthologs to infer their phylogeny and created codon-aware alignments to determine orthologs under selective pressure in our pathogenic species. Our results show several shared BGCs between A. apis, A. aggregata and A. pollenicola, with antifungal resistance related genes present in the bee pathogens and commensals. Genes involved in metabolism and protein processing exhibit signatures of enrichment and positive selection under a fitted branch-site model. Additional known virulence genes in A. pollenicola, A. acerosa and A. atra are identified, supporting previous hypotheses that these commensals may be opportunistic pathogens. Finally, we discuss the importance of such genes in other fungal pathogens, suggesting a common route to evolution of pathogenicity in Ascosphaera.

Indexed as

AscomycotaOnygenalesAnimalsAntifungal AgentsBeesGenomicsPhylogenyAntifungal AgentsBiosynthetic gene clustersChalkbroodEurotiomycetesEvolutionary genomicsFunctional enrichmentPositive selection

Identifiers

PMID35933037
PMCPMC10793876
OpenAlexW4289598267

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.