ArticleBMC veterinary research2022
Isolation and molecular characterization of lumpy skin disease virus from hard ticks, Rhipicephalus (Boophilus) annulatus in Egypt.
Article in BMC veterinary research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 2 of them syntheses that pooled it.
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Who cites it
19 citing papers in PubMed, 2 syntheses or guidelines pooled it, 33 citations in OpenAlex.
- A systematic review of epidemiological modelling in response to lumpy skin disease outbreaks.Frontiers in veterinary science · 2024Pooled it
- Lumpy Skin Disease: A Systematic Review of Mode of Transmission, Risk of Emergence and Risk Entry Pathway.Viruses · 2023Pooled it
- Diagnostic approach, vaccination and treatment priorities for Lumpy Skin Disease in cattle: lessons from Balkan epidemics to Western Europe re-emergence (2025).Veterinary research communications · 2026Review
- Demonstration of the pathogenicity of LSDV pendik strain in cattle.Archives of virology · 2026Article
- MaxEnt-based suitable areas analysis of global lumpy skin disease under current and future climate scenarios.Frontiers in microbiology · 2026Article
- Review
- Global expansion of lumpy skin disease: transmission trends, economic consequences, and preventive measures.Veterinary research communications · 2025Review
- The silent spread: uncovering the diversity and evolution of poxviruses in ticks across Western China's host landscapes.Virology journal · 2025Article
- Evaluation of phytochemicals and essential oils of Cupressus semprevirens in controlling cattle tick Rhipicephalus annulatus (Acari: Ixodidae).BMC plant biology · 2025Article
- Progress in diagnostic methods and vaccines for lumpy skin disease virus: a path towards understanding the disease.Veterinary research communications · 2025Review
- Lumpy skin disease: A growing threat to the global livestock industry.Open veterinary journal · 2025Review
- Genetic Evolutionary Analysis of Lumpy Skin Disease Virus Strain Under Immune Pressure Exerted by Heterologous Goat Poxvirus Vaccines.Transboundary and emerging diseases · 2025Article
- Molecular detection of lumpy skin disease virus in naturally infected cattle and buffaloes: unveiling the role of tick vectors in disease spread.Veterinary research communications · 2024Article
- Lumpy Skin Disease: Insights into Molecular Pathogenesis and Control Strategies.Veterinary sciences · 2024Review
- Article
- Review
- Poxvirus sensitivity of a novel diploid sheep embryonic heart cell line.Archives of virology · 2023Article
- An Extensive Examination of the Warning Signs, Symptoms, Diagnosis, Available Therapies, and Prognosis for Lumpy Skin Disease.Viruses · 2023Review
- Understanding the research advances on lumpy skin disease: A comprehensive literature review of experimental evidence.Frontiers in microbiology · 2022Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundLumpy skin disease (LSD), a disease of cattle and buffaloes, has recently become widely prevalent in Egypt. The aim of this study was to ascertain the potential role of Rhipicephalus (Boophilus) annulatus ticks in the transmission of this disease. Samples collected from suspected lumpy skin disease virus (LSDV) infected cows that had previously been vaccinated with the Romanian sheep pox virus (SPPV) in various Egyptian governorates were obtained between May to November over two consecutive years, namely 2018 and 2019. Ticks were morphologically identified and the partial cytochrome oxidase subunit I gene (COI) were sequenced, revealing that they were closely related to R. (Boophilus) annulatus. The G-protein-coupled chemokine receptor (GPCR) gene of the LSDV was used to test hard ticks.
resultsTwo positive samples from Kafr El-Sheikh province and one positive sample from Al-Behera province were reported. BLAST analysis revealed that the positive samples were closely related to the Kazakhstani Kubash/KAZ/16 strain (accession number MN642592). Phylogenetic analysis revealed that the GPCR gene of the LSDV recently circulating in Egypt belongs to a global cluster of field LSDV with a nucleotide identity of 98-100%. LSDV isolation was successfully performed four days after inoculation using 9 to 11-day-old embryonated chicken eggs showing characteristic focal white pock lesions dispersed on the choriallantoic membrane after three blind passages. Intracytoplasmic inclusion bodies, cell rupture, vacuoles in cells, and virus particles ovoid in shape were demonstrated by electron microscopy.
conclusionIn this study the role of hard ticks in the transmission of the LSDV to susceptible animals in Egypt was revealed and confirmed by various methods.
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