Evidence map›Paper›PMID 35931829›Full record

ArticlePflugers Archiv : European journal of physiology2022

Calpain-dependent degradation of cytoskeletal proteins as a key mechanism for a reduction in intrinsic passive stiffness of unloaded rat postural muscle.

I Y Melnikov, Sergey A Tyganov, K A Sharlo, A D Ulanova, I M Vikhlyantsev, T M Mirzoev, B S Shenkman

Abstract read
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In one paragraph

Article in Pflugers Archiv : European journal of physiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 6 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

I Y MelnikovMyology Laboratory, Institute of Biomedical Problems, Russian Academy of Sciences, 76a Khoroshevskoe shosse, Moscow, Russian Federation.
Sergey A TyganovMyology Laboratory, Institute of Biomedical Problems, Russian Academy of Sciences, 76a Khoroshevskoe shosse, Moscow, Russian Federation. sentackle@yandex.ru.
K A SharloMyology Laboratory, Institute of Biomedical Problems, Russian Academy of Sciences, 76a Khoroshevskoe shosse, Moscow, Russian Federation.
A D UlanovaLaboratory of Structure and Functions of Muscle Proteins, Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia.
I M VikhlyantsevLaboratory of Structure and Functions of Muscle Proteins, Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia.
T M MirzoevMyology Laboratory, Institute of Biomedical Problems, Russian Academy of Sciences, 76a Khoroshevskoe shosse, Moscow, Russian Federation.
B S ShenkmanMyology Laboratory, Institute of Biomedical Problems, Russian Academy of Sciences, 76a Khoroshevskoe shosse, Moscow, Russian Federation.
Institute of Biomedical Problems · RUInstitute of Theoretical and Experimental Biophysics · RU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In mammals, prolonged mechanical unloading results in a significant decrease in passive stiffness of postural muscles. The nature of this phenomenon remains unclear. The aim of the present study was to investigate possible causes for a reduction in rat soleus passive stiffness after 7 and 14 days of unloading (hindlimb suspension, HS). We hypothesized that HS-induced decrease in passive stiffness would be associated with calpain-dependent degradation of cytoskeletal proteins or a decrease in actomyosin interaction. Wistar rats were subjected to HS for 7 and 14 days with or without PD150606 (calpain inhibitor) treatment. Soleus muscles were subjected to biochemical analysis and ex vivo measurements of passive tension with or without blebbistatin treatment (an inhibitor of actomyosin interactions). Passive tension of isolated soleus muscle was significantly reduced after 7- and 14-day HS compared to the control values. PD150606 treatment during 7- and 14-day HS induced an increase in alpha-actinin-2 and -3, desmin contents compared to control, partly prevented a decrease in intact titin (T1) content, and prevented a decrease in soleus passive tension. Incubation of soleus muscle with blebbistatin did not affect HS-induced reductions in specific passive tension in soleus muscle. Our study suggests that calpain-dependent breakdown of cytoskeletal proteins, but not a change in actomyosin interaction, significantly contributes to unloading-induced reductions in intrinsic passive stiffness of rat soleus muscle.

Indexed as

ActomyosinCalpainAcrylatesActininAnimalsConnectinDesminHindlimb SuspensionMammalsMuscle, SkeletalRatsRats, WistarAcrylatesActininActomyosinCalpainConnectinDesminPD 150606BlebbistatinCalpainsCytoskeletal proteinsPassive stiffnessPD150606Soleus muscle

Identifiers

PMID35931829
OpenAlexW4290048247

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.