ArticleBiofabrication2022
Human gelatin-based composite hydrogels for osteochondral tissue engineering and their adaptation into bioinks for extrusion, inkjet, and digital light processing bioprinting.
Article in Biofabrication, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 47 citations in OpenAlex.
- Innovative Hydroxyapatite-Hydrogel Composites for Cartilage Regeneration.Gels (Basel, Switzerland) · 2026Review
- Innovative prospects in 3D printed bio-scaffolds for osteochondral tissue engineering: A systematic review.World journal of methodology · 2026Article
- Natural-Based Nanocomposite Ink Engineering for Seamless Multi-Material Integration in Extrusion-Based 3D Printing.Advanced healthcare materials · 2026Article
- Innovative tissue engineering strategies for auricular regeneration in microtia: Current advances and future perspectives.Regenerative therapy · 2025Review
- Dynamic Hydrogels in Breast Tumor Models.Gels (Basel, Switzerland) · 2025Review
- Strategic advances in Vat Photopolymerization for 3D printing of calcium phosphate-based bone scaffolds: A review.Bioactive materials · 2025Review
- 3D bioprinted scaffolds for osteochondral regeneration: advancements and applications.Materials today. Bio · 2025Review
- Biofabrication of HepG2 Cells-Laden 3D Structures Using Nanocellulose-Reinforced Gelatin-Based Hydrogel Bioinks: Materials Characterization, Cell Viability Assessment, and Metabolomic Analysis.ACS biomaterials science & engineering · 2025Article
- Development of Hydrogels Fabricated via Stereolithography for Bioengineering Applications.Polymers · 2025Review
- Advancements of 3D bioprinting in regenerative medicine: Exploring cell sources for organ fabrication.Heliyon · 2024Review
- Calcium Phosphate Biomaterials for 3D Bioprinting in Bone Tissue Engineering.Biomimetics (Basel, Switzerland) · 2024Review
- Development of Human-Derived Photocrosslinkable Gelatin Hydrogels for Tissue Engineering.Biomacromolecules · 2024Article
- Advances in 3D bioprinting for regenerative medicine applications.Regenerative biomaterials · 2024Review
- Bioprinting of gelatin-based materials for orthopedic application.Frontiers in bioengineering and biotechnology · 2024Review
- 3D printed osteochondral scaffolds: design strategies, present applications and future perspectives.Frontiers in bioengineering and biotechnology · 2024Review
- 3D-Printed Hydrogel for Diverse Applications: A Review.Gels (Basel, Switzerland) · 2023Review
- Collagen and Beyond: A Comprehensive Comparison of Human ECM Properties Derived from Various Tissue Sources for Regenerative Medicine Applications.Journal of functional biomaterials · 2023Article
- Advances in Cartilage Tissue Engineering Using Bioinks with Decellularized Cartilage and Three-Dimensional Printing.International journal of molecular sciences · 2023Review
- Nanocomposite Bioprinting for Tissue Engineering Applications.Gels (Basel, Switzerland) · 2023Review
- Development of photoreactive demineralized bone matrix 3D printing colloidal inks for bone tissue engineering.Regenerative biomaterials · 2023Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
The investigation of novel hydrogel systems allows for the study of relationships between biomaterials, cells, and other factors within osteochondral tissue engineering. Three-dimensional (3D) printing is a popular research method that can allow for further interrogation of these questions via the fabrication of 3D hydrogel environments that mimic tissue-specific, complex architectures. However, the adaptation of promising hydrogel biomaterial systems into 3D-printable bioinks remains a challenge. Here, we delineated an approach to that process. First, we characterized a novel methacryloylated gelatin composite hydrogel system and assessed how calcium phosphate and glycosaminoglycan additives upregulated bone- and cartilage-like matrix deposition and certain genetic markers of differentiation within human mesenchymal stem cells (hMSCs), such as RUNX2 and SOX9. Then, new assays were developed and utilized to study the effects of xanthan gum and nanofibrillated cellulose, which allowed for cohesive fiber deposition, reliable droplet formation, and non-fracturing digital light processing (DLP)-printed constructs within extrusion, inkjet, and DLP techniques, respectively. Finally, these bioinks were used to 3D print constructs containing viable encapsulated hMSCs over a 7 d period, where DLP printed constructs facilitated the highest observed increase in cell number over 7 d (∼2.4×). The results presented here describe the promotion of osteochondral phenotypes via these novel composite hydrogel formulations, establish their ability to bioprint viable, cell-encapsulating constructs using three different 3D printing methods on multiple bioprinters, and document how a library of modular bioink additives affected those physicochemical properties important to printability.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.