Molecular identification and genotyping of Acanthamoeba spp., in bronchoalveolar lavage fluid from immunocompetent patients with chronic respiratory disorders (CRD).

Reza Saberi, Maryam Nakhaei, Mahdi Fakhar, Hossein Zarrinfar, Ali Sharifpour, Hajar Ziaei Hezarjaribi

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Article in Parasitology research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

1.1field-weighted citation impact, top 24% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed, 5 citations in OpenAlex.

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4 · The record

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5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Reza SaberiIranian National Registry Center for Lophomoniasis and Toxoplasmosis, School of Medicine, Imam Khomeini Hospital, Mazandaran University of Medical Sciences, P.O Box: 48471-91971, Sari, Iran.

Maryam NakhaeiIranian National Registry Center for Lophomoniasis and Toxoplasmosis, School of Medicine, Imam Khomeini Hospital, Mazandaran University of Medical Sciences, P.O Box: 48471-91971, Sari, Iran.

Mahdi FakharIranian National Registry Center for Lophomoniasis and Toxoplasmosis, School of Medicine, Imam Khomeini Hospital, Mazandaran University of Medical Sciences, P.O Box: 48471-91971, Sari, Iran. mahdifakhar53@gmail.com.ORCID http://orcid.org/0000-0002-4690-6938

Hossein ZarrinfarDepartment of Medical Mycology and Parasitology, Faculty of Medicine, Allergy Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.

Ali SharifpourIranian National Registry Center for Lophomoniasis and Toxoplasmosis, School of Medicine, Imam Khomeini Hospital, Mazandaran University of Medical Sciences, P.O Box: 48471-91971, Sari, Iran.

Hajar Ziaei HezarjaribiIranian National Registry Center for Lophomoniasis and Toxoplasmosis, School of Medicine, Imam Khomeini Hospital, Mazandaran University of Medical Sciences, P.O Box: 48471-91971, Sari, Iran.

Imam Khomeini Hospital · IRMashhad University of Medical Sciences · IR

Funding

Mazandaran University of Medical Sciences 9241

6 · The paper itself

Abstract

This study aimed to investigate the presence and genotyping of Acanthamoeba spp., in the bronchoalveolar lavage fluid (BALF) of immunocompetent patients with chronic respiratory disorders (CRD). In this study, 211 BALF samples were collected from patients with CRD during the COVID-19 pandemic who were candidates for fiberoptic bronchoscopy (FOB) at Imam Khomeini Hospital, Sari, Mazandaran Province, northern Iran and investigated for Acanthamoeba spp., by PCR. A total of 211 FBAL samples were examined; 5 (5/211; 2.36%) were positive by using the PCR test for Acanthamoeba spp. According to sequence analysis, three strains belonged to the T4 genotype and one strain to the T2 genotype. Our data demonstrate that the presence of Acanthamoeba (T4 and T2) in BALF specimens of patients with respiratory infections. However, it is important to note that these findings may be merely accidental. Our findings suggest further investigation to fully understand the role of Acanthamoeba spp. in the pathogenesis of lung infections.

Indexed as

AcanthamoebaCOVID-19Bronchoalveolar Lavage FluidGenotypeHumansPandemicsRNA, Ribosomal, 18SRNA, Ribosomal, 18SAcanthamoebaBronchoalveolar lavagePCRT2 genotypeT4 genotype

Identifiers

PMID35930044

DOI10.1007/s00436-022-07609-1

PMCPMC9362591

OpenAlexW4289890467

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.